Effect of cholic acid on fetal cardiac myocytes in intrahepatic choliestasis of pregnancy.
J Huazhong Univ Sci Technolog Med Sci
; 34(5): 736-739, 2014 Oct.
Article
in En
| MEDLINE
| ID: mdl-25318885
ABSTRACT
This study examined the effect of cholic acid (CA) on cultured cardiac myocytes (CMs) from neonatal rats with an attempt to explore the possible mechanism of sudden fetal death in intrahepatic cholestasis of pregnancy (ICP). Inverted microscopy was performed to detect the impact of CA on the beating rates of rat CMs. MTT method was used to study the effect of CA on the viability of CMs. CMs cultured in vitro were incubated with 10 µmol/L Ca(2+)-sensitive fluorescence indicator fluo-3/AM. The fluorescence signals of free calcium induced by CA were measured under a laser scanning confocal microscope. The results showed that CA decreased the beating rates of the CMs in a dose-dependent manner. CA could suppress the activities of CMs in a time- and dose-dependent manner. CA increased the concentration of intracellular free calcium in a dose-dependent manner. Our study suggested that CA could inhibit the activity of CMs by causing calcium overload, thereby leading to the sudden fetal death in ICP.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pregnancy Complications
/
Cholestasis, Intrahepatic
/
Cholic Acid
/
Myocytes, Cardiac
Type of study:
Etiology_studies
Limits:
Animals
/
Female
/
Humans
/
Pregnancy
Language:
En
Journal:
J Huazhong Univ Sci Technolog Med Sci
Journal subject:
MEDICINA
Year:
2014
Type:
Article
Affiliation country:
China