Magnetic bead-based isolation of exosomes.
Methods Mol Biol
; 1218: 465-81, 2015.
Article
in En
| MEDLINE
| ID: mdl-25319668
ABSTRACT
Exosomes are here defined as extracellular vesicles (EVs) in the approximate size range of 30-100 nm in diameter, and are observed in most body fluids containing typical exosomal markers such as CD9, CD63, and CD81. Potential subpopulations of exosomes can be captured by targeting these markers using magnetic beads. Magnetic beads are versatile tools for exosome isolation and downstream analysis. Here, we describe the workflow of immuno magnetic isolation and analysis of exosomes by flow cytometry, Western immunoblotting, and electron microscopy.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cell Fractionation
/
Immunomagnetic Separation
/
Exosomes
Limits:
Humans
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2015
Type:
Article
Affiliation country:
Norway