Profiling Chlamydomonas metabolism under dark, anoxic H2-producing conditions using a combined proteomic, transcriptomic, and metabolomic approach.

Subramanian, Venkataramanan; Dubini, Alexandra; Astling, David P; Laurens, Lieve M L; Old, William M; Grossman, Arthur R; Posewitz, Matthew C; Seibert, Michael

Subramanian, Venkataramanan; Dubini, Alexandra; Astling, David P; Laurens, Lieve M L; Old, William M; Grossman, Arthur R; Posewitz, Matthew C; Seibert, Michael.

Affiliation

Subramanian V; National Renewable Energy Laboratory , Golden, Colorado 80401, United States.

J Proteome Res ; 13(12): 5431-51, 2014 Dec 05.

Article in En | MEDLINE | ID: mdl-25333711

Subject(s)

Chlamydomonas reinhardtii/genetics; Chlamydomonas reinhardtii/metabolism; Gene Expression Profiling/methods; Hydrogen/metabolism; Metabolomics/methods; Proteomics/methods; Algal Proteins/genetics; Algal Proteins/metabolism; Amino Acids/metabolism; Anaerobiosis; Blotting, Western; Carbon/metabolism; Darkness; Electrophoresis, Gel, Two-Dimensional; Gas Chromatography-Mass Spectrometry; Mass Spectrometry; Metabolic Networks and Pathways/genetics; Metabolome; Nitrogen/metabolism; Proteome/metabolism; Reverse Transcriptase Polymerase Chain Reaction

Key words

Chlamydomonas reinhardtii; TCA reactions; amino acid synthesis; glyoxylate pathway; hydrogen production; isocitrate lyase; proteomics

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Chlamydomonas reinhardtii / Gene Expression Profiling / Proteomics / Metabolomics / Hydrogen Language: En Journal: J Proteome Res Journal subject: BIOQUIMICA Year: 2014 Type: Article Affiliation country: United States

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