Allosteric activation of ADAMTS13 by von Willebrand factor.
Proc Natl Acad Sci U S A
; 111(52): 18584-9, 2014 Dec 30.
Article
in En
| MEDLINE
| ID: mdl-25512528
ABSTRACT
The metalloprotease ADAMTS13 cleaves von Willebrand factor (VWF) within endovascular platelet aggregates, and ADAMTS13 deficiency causes fatal microvascular thrombosis. The proximal metalloprotease (M), disintegrin-like (D), thrombospondin-1 (T), Cys-rich (C), and spacer (S) domains of ADAMTS13 recognize a cryptic site in VWF that is exposed by tensile force. Another seven T and two complement C1r/C1s, sea urchin epidermal growth factor, and bone morphogenetic protein (CUB) domains of uncertain function are C-terminal to the MDTCS domains. We find that the distal T8-CUB2 domains markedly inhibit substrate cleavage, and binding of VWF or monoclonal antibodies to distal ADAMTS13 domains relieves this autoinhibition. Small angle X-ray scattering data indicate that distal T-CUB domains interact with proximal MDTCS domains. Thus, ADAMTS13 is regulated by substrate-induced allosteric activation, which may optimize VWF cleavage under fluid shear stress in vivo. Distal domains of other ADAMTS proteases may have similar allosteric properties.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Von Willebrand Factor
/
ADAM Proteins
Limits:
Humans
Language:
En
Journal:
Proc Natl Acad Sci U S A
Year:
2014
Type:
Article