Sex identification of ancient DNA samples using a microfluidic device.
Methods Mol Biol
; 1274: 93-8, 2015.
Article
in En
| MEDLINE
| ID: mdl-25673485
ABSTRACT
Ancient DNA is the name given to the degraded, fragmented, and chemically damaged biomolecules that can be recovered from archaeological remains of plants, animals, and humans. Where ancient human DNA has survived at archaeological sites, it can give valuable information and is especially useful for its potential to identify kinship, population affinities, pathogens, and biological sex. Here, we describe the operation of a microfluidic device for the sex identification of ancient DNA samples using an efficient sample handling process. DNA is extracted from powdered bone samples and abasic sites labeled with biotin. Streptavidin-coated superparamagnetic particles are used to isolate the labeled DNA prior to amplification of the Amelogenin sex marker.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
DNA
/
DNA Fingerprinting
/
Microfluidic Analytical Techniques
Type of study:
Diagnostic_studies
Limits:
Animals
/
Female
/
Humans
/
Male
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2015
Type:
Article