Use of HPLC/UPLC-spectrophotometry for detection of formazan in in vitro Reconstructed human Tissue (RhT)-based test methods employing the MTT-reduction assay to expand their applicability to strongly coloured test chemicals.

Alépée, N; Barroso, J; De Smedt, A; De Wever, B; Hibatallah, J; Klaric, M; Mewes, K R; Millet, M; Pfannenbecker, U; Tailhardat, M; Templier, M; McNamee, P

Alépée, N; Barroso, J; De Smedt, A; De Wever, B; Hibatallah, J; Klaric, M; Mewes, K R; Millet, M; Pfannenbecker, U; Tailhardat, M; Templier, M; McNamee, P.

Affiliation

Alépée N; L'Oréal Research & Innovation, Aulnay Sous Bois, France.
Barroso J; Cosmetics Europe, The Personal Care Association, Brussels, Belgium.
De Smedt A; Janssen Research & Development, Beerse, Belgium.
De Wever B; Henkel AG & Co. KGaA, Düsseldorf, Germany.
Hibatallah J; Chanel Parfums Beauté, Neuilly sur Seine, France.
Klaric M; Cosmetics Europe, The Personal Care Association, Brussels, Belgium.
Mewes KR; Henkel AG & Co. KGaA, Düsseldorf, Germany.
Millet M; Laboratoire Pierre Fabre, Castres, France.
Pfannenbecker U; Beiersdorf AG, Hamburg, Germany.
Tailhardat M; LVMH Recherche, St Jean De Braye Cedex, France.
Templier M; Laboratoire Pierre Fabre, Castres, France.
McNamee P; The Procter & Gamble Company, Egham, Surrey, United Kingdom. Electronic address: mcnamee.pm@pg.com.

Toxicol In Vitro ; 29(4): 741-61, 2015 Jun.

Article in En | MEDLINE | ID: mdl-25701760

Subject(s)

Coloring Agents/toxicity; Formazans/toxicity; Skin Irritancy Tests/methods; Animal Testing Alternatives; Chromatography, High Pressure Liquid; Cosmetics/toxicity; Eye Diseases/chemically induced; Humans; Irritants/toxicity; Reproducibility of Results; Skin Diseases/chemically induced; Skin Diseases/pathology; Spectrophotometry, Ultraviolet; Tetrazolium Salts/chemistry; Thiazoles/chemistry

Key words

Eye irritation; HPLC/UPLC-spectrophotometry; In vitro RhT test methods; Skin corrosion; Skin irritation

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Skin Irritancy Tests / Coloring Agents / Formazans Type of study: Diagnostic_studies Limits: Humans Language: En Journal: Toxicol In Vitro Journal subject: TOXICOLOGIA Year: 2015 Type: Article Affiliation country: France

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