Contemporary techniques for freezing mouse spermatozoa.
Curr Protoc Mouse Biol
; 4(3): 85-104, 2014 Sep 03.
Article
in En
| MEDLINE
| ID: mdl-25723962
ABSTRACT
Each year, thousands of new mouse models are generated around the world to further biomedical research. Unfortunately, the cost of maintaining mouse colonies makes it uneconomical to keep strains on the shelf that are not part of active research programs. Ideally, these retired strains should be archived. If this is not done and the line is simply killed off, the genetics are lost to future generations of scientists. Traditionally, embryo freezing has been used to cryopreserve mice, but this is expensive, time consuming, requires large numbers of donor females, and usually involves invasive superovulation procedures. Sperm freezing circumvents all of these disadvantages and is rapidly becoming the technique of choice for many repositories. This has been made possible through the use of refined cryoprotective agents and the development of improved in vitro fertilization techniques. This article describes two popular sperm freezing techniques employed by mouse repositories to archive spermatozoa using cryoprotective agents supplemented with either L-glutamine or monothioglycerol.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Semen Preservation
/
Spermatozoa
/
Cryopreservation
/
Cryoprotective Agents
/
Glutamine
/
Glycerol
Limits:
Animals
/
Humans
/
Male
Language:
En
Journal:
Curr Protoc Mouse Biol
Year:
2014
Type:
Article
Affiliation country:
United kingdom