Indirect protein quantification of drug-transforming enzymes using peptide group-specific immunoaffinity enrichment and mass spectrometry.
Sci Rep
; 5: 8759, 2015 Mar 04.
Article
in En
| MEDLINE
| ID: mdl-25737130
ABSTRACT
Immunoaffinity enrichment of proteotypic peptides, coupled with selected reaction monitoring, enables indirect protein quantification. However the lack of suitable antibodies limits its widespread application. We developed a method in which multi-specific antibodies are used to enrich groups of peptides, thus facilitating multiplexed quantitative protein assays. We tested this strategy in a pharmacokinetic experiment by targeting a group of homologous drug transforming proteins in human hepatocytes. Our results indicate the generic applicability of this method to any biological system.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Peptides
/
Mass Spectrometry
/
Hepatocytes
Limits:
Humans
Language:
En
Journal:
Sci Rep
Year:
2015
Type:
Article
Affiliation country:
Germany