Epicutaneous Immunization with Collagen Induces TCRαß Suppressor T Cells That Inhibit Collagen-Induced Arthritis.
Int Arch Allergy Immunol
; 166(2): 121-34, 2015.
Article
in En
| MEDLINE
| ID: mdl-25824601
ABSTRACT
BACKGROUND:
We have shown previously, in an animal model of multiple sclerosis and in TNBS-induced colitis, that epicutaneous (EC) immunization with protein antigen induces T suppressor cells that strongly inhibit the inflammatory response in contact hypersensitivity reactions.METHODS:
EC immunization was performed by applying to the shaved skin of the mouse dorsum a gauze patch soaked with a solution containing various amounts of type II collagen (COLL II) in a volume of 100 µl of PBS on days 0 and 4. On day 7 the patches were removed and mice were intradermally (i.d.) immunized with COLL II to induce collagen-induced arthritis (CIA).RESULTS:
Our study shows that EC immunization with 100 or 30 µg of COLL II reduces disease severity, whereas lower doses (10 or 3 µg) do not affect CIA. Decreased disease severity observed after EC immunization with COLL II correlates with reduced myeloperoxidase activity in joint tissue and with reduced production of anti-citrullinated protein and anti-COLL II IgG2a antibodies. Transfer experiments show that EC immunization with COLL II induces suppressor cells that belong to the population of TCRαß lymphocytes and that EC-induced suppression declines with time. Both in vitro and in vivo experiments show that IL-17A plays an important role in EC-induced suppression of CIA. EC application of COLL II at the first signs of CIA also results in disease suppression.CONCLUSIONS:
The suppression of inflammatory responses by T suppressor cells induced through EC immunization of a protein antigen may become an attractive noninvasive therapeutic method for a variety of clinical situations.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Arthritis, Experimental
/
Receptors, Antigen, T-Cell, alpha-beta
/
CD8-Positive T-Lymphocytes
/
Collagen Type II
Limits:
Animals
Language:
En
Journal:
Int Arch Allergy Immunol
Journal subject:
ALERGIA E IMUNOLOGIA
Year:
2015
Type:
Article
Affiliation country:
Poland