Purification, cloning, and expression of ciliary neurotrophic factor (CNTF).

Lin, L F; Mismer, D; Lile, J D; Armes, L G; Butler, E T; Vannice, J L; Collins, F

Lin, L F; Mismer, D; Lile, J D; Armes, L G; Butler, E T; Vannice, J L; Collins, F.

Affiliation

Lin LF; Protein Chemistry Group, Synergen, Inc., Boulder, CO 80301.

Science ; 246(4933): 1023-5, 1989 Nov 24.

Article in En | MEDLINE | ID: mdl-2587985

ABSTRACT

ABSTRACT

Ciliary neurotrophic factor (CNTF) is one of a small number of proteins with neurotrophic activities distinct from nerve growth factor (NGF). CNTF has now been purified and cloned and the primary structure of CNTF from rabbit sciatic nerve has been determined. Biologically active CNTF has been transiently expressed from a rabbit complementary DNA clone. CNTF is a neural effector without significant sequence homologies to any previously reported protein.

Subject(s)

Nerve Growth Factors/genetics; Nerve Tissue Proteins/genetics; Amino Acid Sequence; Animals; Cell Line; Ciliary Neurotrophic Factor; Cloning, Molecular; DNA/genetics; Molecular Sequence Data; Nerve Tissue Proteins/biosynthesis; Nerve Tissue Proteins/isolation & purification; Rabbits; Recombinant Proteins/biosynthesis; Sciatic Nerve/metabolism; Transfection

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Collection: 01-internacional Database: MEDLINE Main subject: Nerve Growth Factors / Nerve Tissue Proteins Limits: Animals Language: En Journal: Science Year: 1989 Type: Article

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