Fact versus artifact: avoiding erroneous estimates of sulfated glycosaminoglycan content using the dimethylmethylene blue colorimetric assay for tissue-engineered constructs.

Zheng, C H; Levenston, M E

Zheng, C H; Levenston, M E.

Affiliation

Zheng CH; Department of Mechanical Engineering, Stanford University, Stanford, CA, 94305-4038, USA.levenston@stanford.edu.

Eur Cell Mater ; 29: 224-36; discussion 236, 2015 Apr 19.

Article in En | MEDLINE | ID: mdl-25890595

Subject(s)

Cartilage/metabolism; Colorimetry/methods; Glycosaminoglycans/metabolism; Methylene Blue/analogs & derivatives; Tissue Engineering/methods; Adipose Tissue/cytology; Animals; Artifacts; Cartilage/cytology; Cattle; Cells, Cultured; Chondrocytes/cytology; Chondrocytes/metabolism; Humans; Hyaluronic Acid/metabolism; Hydrogen-Ion Concentration; Menisci, Tibial/cytology; Menisci, Tibial/metabolism; Methylene Blue/metabolism; Nucleic Acids/metabolism; Reproducibility of Results; Stem Cells/cytology; Stem Cells/metabolism

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Cartilage / Colorimetry / Tissue Engineering / Glycosaminoglycans / Methylene Blue Limits: Animals / Humans Language: En Journal: Eur Cell Mater Year: 2015 Type: Article

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