Rapid detection of HLA-B*51 by real-time polymerase chain reaction and high-resolution melting analysis.

Imperiali, C; Alía-Ramos, P; Padró-Miquel, A

Imperiali, C; Alía-Ramos, P; Padró-Miquel, A.

Affiliation

Imperiali C; Genètica Molecular-Laboratori Clínic, Hospital Universitari de Bellvitge, L'Hospitalet de Llobregat, Catalonia, Spain.
Alía-Ramos P; Genètica Molecular-Laboratori Clínic, Hospital Universitari de Bellvitge, L'Hospitalet de Llobregat, Catalonia, Spain.
Padró-Miquel A; Genètica Molecular-Laboratori Clínic, Hospital Universitari de Bellvitge, L'Hospitalet de Llobregat, Catalonia, Spain.

Tissue Antigens ; 86(2): 139-42, 2015 Aug.

Article in En | MEDLINE | ID: mdl-26176813

Subject(s)

Behcet Syndrome/diagnosis; Biological Assay/standards; DNA Primers/chemistry; Genotyping Techniques/standards; HLA-B51 Antigen/genetics; Nucleic Acid Amplification Techniques/standards; Behcet Syndrome/genetics; Behcet Syndrome/immunology; Biological Assay/economics; Biological Assay/instrumentation; Genotype; Genotyping Techniques/economics; Genotyping Techniques/instrumentation; HLA-B51 Antigen/immunology; Humans; Nucleic Acid Amplification Techniques/economics; Nucleic Acid Amplification Techniques/instrumentation; Nucleic Acid Denaturation; Reproducibility of Results; Sensitivity and Specificity; Time Factors

Key words

Behçet disease; HLA-B*51; high-resolution melting assay; method; real-time PCR

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Biological Assay / Behcet Syndrome / DNA Primers / Nucleic Acid Amplification Techniques / HLA-B51 Antigen / Genotyping Techniques Type of study: Diagnostic_studies / Prognostic_studies / Risk_factors_studies Limits: Humans Language: En Journal: Tissue Antigens Year: 2015 Type: Article Affiliation country: Spain

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