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E-cadherin junction formation involves an active kinetic nucleation process.
Biswas, Kabir H; Hartman, Kevin L; Yu, Cheng-han; Harrison, Oliver J; Song, Hang; Smith, Adam W; Huang, William Y C; Lin, Wan-Chen; Guo, Zhenhuan; Padmanabhan, Anup; Troyanovsky, Sergey M; Dustin, Michael L; Shapiro, Lawrence; Honig, Barry; Zaidel-Bar, Ronen; Groves, Jay T.
Affiliation
  • Biswas KH; Mechanobiology Institute, National University of Singapore, Singapore 117411;
  • Hartman KL; Mechanobiology Institute, National University of Singapore, Singapore 117411; Department of Chemistry, University of California, Berkeley, CA 94720;
  • Yu CH; Mechanobiology Institute, National University of Singapore, Singapore 117411;
  • Harrison OJ; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032; Howard Hughes Medical Institute, Columbia University, New York, NY 10032; Center for Computational Biology and Bioinformatics, Columbia University, New York, NY 10032;
  • Song H; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032; Howard Hughes Medical Institute, Columbia University, New York, NY 10032; Center for Computational Biology and Bioinformatics, Columbia University, New York, NY 10032;
  • Smith AW; Howard Hughes Medical Institute, University of California, Berkeley, CA 94720;
  • Huang WY; Department of Chemistry, University of California, Berkeley, CA 94720;
  • Lin WC; Howard Hughes Medical Institute, University of California, Berkeley, CA 94720;
  • Guo Z; Mechanobiology Institute, National University of Singapore, Singapore 117411;
  • Padmanabhan A; Mechanobiology Institute, National University of Singapore, Singapore 117411;
  • Troyanovsky SM; Department of Dermatology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611;
  • Dustin ML; Kennedy Institute, Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, University of Oxford, Headington OX3 7FY, United Kingdom;
  • Shapiro L; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032; Center for Computational Biology and Bioinformatics, Columbia University, New York, NY 10032;
  • Honig B; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032; Howard Hughes Medical Institute, Columbia University, New York, NY 10032; Center for Computational Biology and Bioinformatics, Columbia University, New York, NY 10032; bh6@cumc.columbia.edu biezbr@nus.edu.s
  • Zaidel-Bar R; Mechanobiology Institute, National University of Singapore, Singapore 117411; Department of Biomedical Engineering, National University of Singapore, Singapore 117411; bh6@cumc.columbia.edu biezbr@nus.edu.sg jtgroves@lbl.gov.
  • Groves JT; Mechanobiology Institute, National University of Singapore, Singapore 117411; Department of Chemistry, University of California, Berkeley, CA 94720; Howard Hughes Medical Institute, University of California, Berkeley, CA 94720; Physical Biosciences and Materials Sciences Divisions, Lawrence Berkeley
Proc Natl Acad Sci U S A ; 112(35): 10932-7, 2015 Sep 01.
Article in En | MEDLINE | ID: mdl-26290581
Epithelial (E)-cadherin-mediated cell-cell junctions play important roles in the development and maintenance of tissue structure in multicellular organisms. E-cadherin adhesion is thus a key element of the cellular microenvironment that provides both mechanical and biochemical signaling inputs. Here, we report in vitro reconstitution of junction-like structures between native E-cadherin in living cells and the extracellular domain of E-cadherin (E-cad-ECD) in a supported membrane. Junction formation in this hybrid live cell-supported membrane configuration requires both active processes within the living cell and a supported membrane with low E-cad-ECD mobility. The hybrid junctions recruit α-catenin and exhibit remodeled cortical actin. Observations suggest that the initial stages of junction formation in this hybrid system depend on the trans but not the cis interactions between E-cadherin molecules, and proceed via a nucleation process in which protrusion and retraction of filopodia play a key role.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Cadherins / Intercellular Junctions Limits: Humans Language: En Journal: Proc Natl Acad Sci U S A Year: 2015 Type: Article
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Cadherins / Intercellular Junctions Limits: Humans Language: En Journal: Proc Natl Acad Sci U S A Year: 2015 Type: Article