Atractylenolide I protects mice from lipopolysaccharide-induced acute lung injury.
Eur J Pharmacol
; 765: 94-9, 2015 Oct 15.
Article
in En
| MEDLINE
| ID: mdl-26297303
ABSTRACT
Atractylenolide I (AO-I), one of the major bioactive components isolated from Rhizoma Atractylodes macrocephala, has been reported to have anti-inflammatory effects. In the present study, we investigated the protective effects of AO-I on acute lung injury (ALI) using LPS-induced ALI mouse model. Lung injury was assessed by histological study. Inflammatory cytokines TNF-α, IL-6 and IL-1ß production were detected by ELISA. TLR4 expression and NF-κB activation were measured by western blot analysis. The results showed that treatment of AO-I significantly attenuated LPS-induced lung wet-to-dry weight ratio and MPO activity. Meanwhile, treatment of AO-I significantly inhibited the production of TNF-α, IL-6, IL-1ß, IL-13, and MIF production in bronchoalveolar lavage fluid (BALF), as well as neutrophils and macrophages in BALF. AO-1 could up-regulate the production of IL-10 in BALF. Besides, LPS-induced TLR4 expression and NF-κB activation were suppressed by treatment of AO-I. In conclusion, the current study suggested that AO-I protected mice acute lung injury induced by LPS via inhibition of TLR4 expression and NF-κB activation.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Sesquiterpenes
/
Lipopolysaccharides
/
Inflammation Mediators
/
Acute Lung Injury
/
Lactones
Limits:
Animals
Language:
En
Journal:
Eur J Pharmacol
Year:
2015
Type:
Article
Affiliation country:
China