A molecular fluorescent dye for specific staining and imaging of RNA in live cells: a novel ligand integration from classical thiazole orange and styryl compounds.

Lu, Yu-Jing; Deng, Qiang; Hu, Dong-Ping; Wang, Zheng-Ya; Huang, Bao-Hua; Du, Zhi-Yun; Fang, Yan-Xiong; Wong, Wing-Leung; Zhang, Kun; Chow, Cheuk-Fai

Lu, Yu-Jing; Deng, Qiang; Hu, Dong-Ping; Wang, Zheng-Ya; Huang, Bao-Hua; Du, Zhi-Yun; Fang, Yan-Xiong; Wong, Wing-Leung; Zhang, Kun; Chow, Cheuk-Fai.

Affiliation

Lu YJ; Institute of Natural Medicine and Green Chemistry, School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, P. R. China. luyj@gdut.edu.cn kzhang@gdut.edu.cn.

Chem Commun (Camb) ; 51(83): 15241-4, 2015 Oct 25.

Article in En | MEDLINE | ID: mdl-26329127

ABSTRACT

A new RNA-selective fluorescent dye integrated with a thiazole orange and a p-(methylthio)styryl moiety shows better nucleolus RNA staining and imaging performance in live cells than the commercial stains. It also exhibits excellent photostability, cell tolerance, and counterstain compatibility with 4',6-diamidino-2-phenylindole for specific RNA-DNA colocalization in bioassays.

Subject(s)

Benzothiazoles/chemistry; Fluorescent Dyes/chemistry; Quinolines/chemistry; RNA, Neoplasm/analysis; Styrenes/chemistry; Animals; Cell Line; Humans; Ligands; Mice; Molecular Structure; NIH 3T3 Cells

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Quinolines / Styrenes / RNA, Neoplasm / Benzothiazoles / Fluorescent Dyes Limits: Animals / Humans Language: En Journal: Chem Commun (Camb) Journal subject: QUIMICA Year: 2015 Type: Article

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