Direct production of functional matrix metalloproteinase--14 without refolding or activation and its application for in vitro inhibition assays.

Nam, Dong Hyun; Ge, Xin

Nam, Dong Hyun; Ge, Xin.

Affiliation

Nam DH; Department of Chemical and Environmental Engineering, University of California, 900 University Ave Riverside, Riverside, 92521, California.
Ge X; Department of Chemical and Environmental Engineering, University of California, 900 University Ave Riverside, Riverside, 92521, California. xge@engr.ucr.edu.

Biotechnol Bioeng ; 113(4): 717-23, 2016 Apr.

Article in En | MEDLINE | ID: mdl-26416249

Subject(s)

Escherichia coli/metabolism; Matrix Metalloproteinase 14/metabolism; Recombinant Proteins/metabolism; Antineoplastic Agents/isolation & purification; Drug Evaluation, Preclinical/methods; Enzyme Inhibitors/isolation & purification; Escherichia coli/genetics; Humans; Matrix Metalloproteinase 14/genetics; Recombinant Proteins/genetics

Key words

MMP; inhibitory antibody; periplasmic expression

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Recombinant Proteins / Escherichia coli / Matrix Metalloproteinase 14 Limits: Humans Language: En Journal: Biotechnol Bioeng Year: 2016 Type: Article

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