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Detection of Airway Colonization by Aspergillus fumigatus by Use of Electronic Nose Technology in Patients with Cystic Fibrosis.
de Heer, K; Kok, M G M; Fens, N; Weersink, E J M; Zwinderman, A H; van der Schee, M P C; Visser, C E; van Oers, M H J; Sterk, P J.
Affiliation
  • de Heer K; Department of Hematology, Academic Medical Center, Amsterdam, the Netherlands koen@de-heer.eu.
  • Kok MG; Department of Hematology, Academic Medical Center, Amsterdam, the Netherlands.
  • Fens N; Department of Respiratory Medicine, Academic Medical Center, Amsterdam, the Netherlands.
  • Weersink EJ; Department of Respiratory Medicine, Academic Medical Center, Amsterdam, the Netherlands.
  • Zwinderman AH; Department of Clinical Epidemiology, Biostatistics, and Bioinformatics, Academic Medical Center, Amsterdam, the Netherlands.
  • van der Schee MP; Department of Respiratory Medicine, Academic Medical Center, Amsterdam, the Netherlands.
  • Visser CE; Department of Medical Microbiology, Academic Medical Center, Amsterdam, the Netherlands.
  • van Oers MH; Department of Hematology, Academic Medical Center, Amsterdam, the Netherlands.
  • Sterk PJ; Department of Respiratory Medicine, Academic Medical Center, Amsterdam, the Netherlands.
J Clin Microbiol ; 54(3): 569-75, 2016 Mar.
Article in En | MEDLINE | ID: mdl-26677251
ABSTRACT
Currently, there is no noninvasive test that can reliably diagnose early invasive pulmonary aspergillosis (IA). An electronic nose (eNose) can discriminate various lung diseases through an analysis of exhaled volatile organic compounds. We recently published a proof-of-principle study showing that patients with prolonged chemotherapy-induced neutropenia and IA have a distinct exhaled breath profile (or breathprint) that can be discriminated with an eNose. An eNose is cheap and noninvasive, and it yields results within minutes. We determined whether Aspergillus fumigatus colonization may also be detected with an eNose in cystic fibrosis (CF) patients. Exhaled breath samples of 27 CF patients were analyzed with a Cyranose 320. Culture of sputum samples defined the A. fumigatus colonization status. eNose data were classified using canonical discriminant analysis after principal component reduction. Our primary outcome was cross-validated accuracy, defined as the percentage of correctly classified subjects using the leave-one-out method. The P value was calculated by the generation of 100,000 random alternative classifications. Nine of the 27 subjects were colonized by A. fumigatus. In total, 3 subjects were misclassified, resulting in a cross-validated accuracy of the Cyranose detecting IA of 89% (P = 0.004; sensitivity, 78%; specificity, 94%). Receiver operating characteristic (ROC) curve analysis showed an area under the curve (AUC) of 0.89. The results indicate that A. fumigatus colonization leads to a distinctive breathprint in CF patients. The present proof-of-concept data merit external validation and monitoring studies.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Aspergillus fumigatus / Breath Tests / Cystic Fibrosis / Invasive Pulmonary Aspergillosis / Electronic Nose Type of study: Diagnostic_studies / Prognostic_studies / Screening_studies Limits: Adolescent / Adult / Female / Humans / Male / Middle aged Language: En Journal: J Clin Microbiol Year: 2016 Type: Article Affiliation country: Netherlands

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Aspergillus fumigatus / Breath Tests / Cystic Fibrosis / Invasive Pulmonary Aspergillosis / Electronic Nose Type of study: Diagnostic_studies / Prognostic_studies / Screening_studies Limits: Adolescent / Adult / Female / Humans / Male / Middle aged Language: En Journal: J Clin Microbiol Year: 2016 Type: Article Affiliation country: Netherlands