Prenucleosomes and Active Chromatin.
Cold Spring Harb Symp Quant Biol
; 80: 65-72, 2015.
Article
in En
| MEDLINE
| ID: mdl-26767995
ABSTRACT
Chromatin consists of nucleosomes as well as nonnucleosomal histone-containing particles. Here we describe the prenucleosome, which is a stable conformational isomer of the nucleosome that associates with â¼80 bp DNA. Prenucleosomes are formed rapidly upon the deposition of histones onto DNA and can be converted into canonical nucleosomes by an ATP-driven chromatin assembly factor such as ACF. Different lines of evidence reveal that there are prenucleosome-sized DNA-containing particles with histones in the upstream region of active promoters. Moreover, p300 acetylates histone H3K56 in prenucleosomes but not in nucleosomes, and H3K56 acetylation is found at active promoters and enhancers. These findings therefore suggest that there may be prenucleosomes or prenucleosome-like particles in the upstream region of active promoters. More generally, we postulate that prenucleosomes or prenucleosome-like particles are present at dynamic chromatin, whereas canonical nucleosomes are at static chromatin.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Chromatin
/
Histones
/
Nucleosomes
Type of study:
Prognostic_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
Cold Spring Harb Symp Quant Biol
Year:
2015
Type:
Article