Harvesting epithelial keratinocyte sheets from temperature-responsive dishes preserves basement membrane proteins and improves cell survival in a skin defect model.

Osada, A; Sekine, H; Soejima, K; Sakurai, H; Shimizu, T

Osada, A; Sekine, H; Soejima, K; Sakurai, H; Shimizu, T.

Affiliation

Osada A; Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University (TWIns), Japan.
Sekine H; Department of Plastic and Reconstructive Surgery, Tokyo Women's Medical University, Japan.
Soejima K; Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University (TWIns), Japan.
Sakurai H; Department of Plastic and Reconstructive Surgery, Nihon University School of Medicine, Tokyo, Japan.
Shimizu T; Department of Plastic and Reconstructive Surgery, Tokyo Women's Medical University, Japan.

J Tissue Eng Regen Med ; 11(9): 2516-2524, 2017 09.

Article in En | MEDLINE | ID: mdl-27061496

Subject(s)

Basement Membrane/metabolism; Cell Separation/methods; Extracellular Matrix Proteins/metabolism; Keratinocytes; Skin; Animals; Cell Survival; Keratinocytes/metabolism; Keratinocytes/transplantation; Rats; Rats, Nude; Rats, Sprague-Dawley; Rats, Transgenic; Skin/injuries; Skin/metabolism; Skin/pathology

Key words

basement membrane; cultured epithelial autograft; dispase; survival rate; temperature-responsive culture dish; transplantation

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Skin / Basement Membrane / Keratinocytes / Cell Separation / Extracellular Matrix Proteins Limits: Animals Language: En Journal: J Tissue Eng Regen Med Journal subject: BIOTECNOLOGIA / HISTOLOGIA Year: 2017 Type: Article Affiliation country: Japan

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