RNA-Seq following PCR-based sorting reveals rare cell transcriptional signatures.

Pellegrino, Maurizio; Sciambi, Adam; Yates, Jamie L; Mast, Joshua D; Silver, Charles; Eastburn, Dennis J

Pellegrino, Maurizio; Sciambi, Adam; Yates, Jamie L; Mast, Joshua D; Silver, Charles; Eastburn, Dennis J.

Affiliation

Pellegrino M; Mission Bio, Inc., 953 Indiana St., San Francisco, California, 94107, USA.
Sciambi A; Mission Bio, Inc., 953 Indiana St., San Francisco, California, 94107, USA.
Yates JL; Mission Bio, Inc., 953 Indiana St., San Francisco, California, 94107, USA.
Mast JD; Mission Bio, Inc., 953 Indiana St., San Francisco, California, 94107, USA.
Silver C; Mission Bio, Inc., 953 Indiana St., San Francisco, California, 94107, USA.
Eastburn DJ; Mission Bio, Inc., 953 Indiana St., San Francisco, California, 94107, USA. eastburn@missionbio.com.

BMC Genomics ; 17: 361, 2016 05 17.

Article in En | MEDLINE | ID: mdl-27189161

Subject(s)

Gene Expression Profiling; High-Throughput Nucleotide Sequencing; Polymerase Chain Reaction; Transcriptome; Cell Line; Cluster Analysis; Gene Expression Profiling/methods; Gene Expression Regulation; High-Throughput Nucleotide Sequencing/methods; Humans; Multiplex Polymerase Chain Reaction; Real-Time Polymerase Chain Reaction; Reproducibility of Results; Sensitivity and Specificity; Single-Cell Analysis/methods; Workflow

Key words

Cell sorting; Droplets; Gene expression; Heterogeneity; Microfluidics; PCR; Single-cell; Transcriptome

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Polymerase Chain Reaction / Gene Expression Profiling / High-Throughput Nucleotide Sequencing / Transcriptome Type of study: Diagnostic_studies / Prognostic_studies Limits: Humans Language: En Journal: BMC Genomics Journal subject: GENETICA Year: 2016 Type: Article Affiliation country: United States

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