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IRF8 Transcription Factor Controls Survival and Function of Terminally Differentiated Conventional and Plasmacytoid Dendritic Cells, Respectively.
Sichien, Dorine; Scott, Charlotte L; Martens, Liesbet; Vanderkerken, Matthias; Van Gassen, Sofie; Plantinga, Maud; Joeris, Thorsten; De Prijck, Sofie; Vanhoutte, Leen; Vanheerswynghels, Manon; Van Isterdael, Gert; Toussaint, Wendy; Madeira, Filipe Branco; Vergote, Karl; Agace, William W; Clausen, Björn E; Hammad, Hamida; Dalod, Marc; Saeys, Yvan; Lambrecht, Bart N; Guilliams, Martin.
Affiliation
  • Sichien D; Lab of Immunoregulation, VIB Inflammation Research Center, Ghent University, 9052 Ghent, Belgium; Department of Biomedical Molecular Biology, Ghent University, 9052 Ghent, Belgium.
  • Scott CL; Lab of Immunoregulation, VIB Inflammation Research Center, Ghent University, 9052 Ghent, Belgium; Department of Biomedical Molecular Biology, Ghent University, 9052 Ghent, Belgium.
  • Martens L; Department of Respiratory Medicine, University Hospital Ghent, 9000 Ghent, Belgium; Data Mining and Modelling for Biomedicine Group, VIB Inflammation Research Center, Ghent University, 9052 Ghent, Belgium.
  • Vanderkerken M; Lab of Immunoregulation, VIB Inflammation Research Center, Ghent University, 9052 Ghent, Belgium; Department of Respiratory Medicine, University Hospital Ghent, 9000 Ghent, Belgium.
  • Van Gassen S; Lab of Immunoregulation, VIB Inflammation Research Center, Ghent University, 9052 Ghent, Belgium; Data Mining and Modelling for Biomedicine Group, VIB Inflammation Research Center, Ghent University, 9052 Ghent, Belgium; Department of Information Technology, iMinds, Ghent University, Ghent, Belgium.
  • Plantinga M; Lab of Immunoregulation, VIB Inflammation Research Center, Ghent University, 9052 Ghent, Belgium; Department of Respiratory Medicine, University Hospital Ghent, 9000 Ghent, Belgium.
  • Joeris T; Section of Immunology and Vaccinology, Danish Technical University Veterinary Institute, 1870 Copenhagen, Denmark.
  • De Prijck S; Lab of Immunoregulation, VIB Inflammation Research Center, Ghent University, 9052 Ghent, Belgium; Department of Biomedical Molecular Biology, Ghent University, 9052 Ghent, Belgium.
  • Vanhoutte L; Lab of Immunoregulation, VIB Inflammation Research Center, Ghent University, 9052 Ghent, Belgium; Department of Medical Genetics, University Hospital Ghent, 9000 Ghent, Belgium.
  • Vanheerswynghels M; Lab of Immunoregulation, VIB Inflammation Research Center, Ghent University, 9052 Ghent, Belgium; Department of Respiratory Medicine, University Hospital Ghent, 9000 Ghent, Belgium.
  • Van Isterdael G; Lab of Immunoregulation, VIB Inflammation Research Center, Ghent University, 9052 Ghent, Belgium; Department of Respiratory Medicine, University Hospital Ghent, 9000 Ghent, Belgium.
  • Toussaint W; Lab of Immunoregulation, VIB Inflammation Research Center, Ghent University, 9052 Ghent, Belgium; Department of Respiratory Medicine, University Hospital Ghent, 9000 Ghent, Belgium.
  • Madeira FB; Lab of Immunoregulation, VIB Inflammation Research Center, Ghent University, 9052 Ghent, Belgium; Department of Respiratory Medicine, University Hospital Ghent, 9000 Ghent, Belgium.
  • Vergote K; Lab of Immunoregulation, VIB Inflammation Research Center, Ghent University, 9052 Ghent, Belgium; Department of Respiratory Medicine, University Hospital Ghent, 9000 Ghent, Belgium.
  • Agace WW; Section of Immunology and Vaccinology, Danish Technical University Veterinary Institute, 1870 Copenhagen, Denmark; Department of Experimental Medical Science, Immunology Section, Lund University, 221 00 Lund, Sweden.
  • Clausen BE; Institute for Molecular Medicine, University Medical Center of the Johannes Gutenberg-University Mainz, 55131 Mainz, Germany.
  • Hammad H; Lab of Immunoregulation, VIB Inflammation Research Center, Ghent University, 9052 Ghent, Belgium; Department of Respiratory Medicine, University Hospital Ghent, 9000 Ghent, Belgium.
  • Dalod M; Centre d'Immunologie de Marseille-Luminy, UNIV UM2, Aix-Marseille Université, Parc Scientifique et Technologique de Luminy, 13288 Marseille, France; INSERM, U1104, 13288 Marseille, France; CNRS, UMR7280, 13288 Marseille, France.
  • Saeys Y; Lab of Immunoregulation, VIB Inflammation Research Center, Ghent University, 9052 Ghent, Belgium; Department of Respiratory Medicine, University Hospital Ghent, 9000 Ghent, Belgium; Data Mining and Modelling for Biomedicine Group, VIB Inflammation Research Center, Ghent University, 9052 Ghent, Belgi
  • Lambrecht BN; Lab of Immunoregulation, VIB Inflammation Research Center, Ghent University, 9052 Ghent, Belgium; Department of Respiratory Medicine, University Hospital Ghent, 9000 Ghent, Belgium; Department of Pulmonary Medicine, Erasmus MC, University Medical Center, Rotterdam, 3015 the Netherlands. Electronic a
  • Guilliams M; Lab of Immunoregulation, VIB Inflammation Research Center, Ghent University, 9052 Ghent, Belgium; Department of Biomedical Molecular Biology, Ghent University, 9052 Ghent, Belgium. Electronic address: martin.guilliams@irc.vib-ugent.be.
Immunity ; 45(3): 626-640, 2016 09 20.
Article in En | MEDLINE | ID: mdl-27637148
Interferon regulatory factor-8 (IRF8) has been proposed to be essential for development of monocytes, plasmacytoid dendritic cells (pDCs) and type 1 conventional dendritic cells (cDC1s) and remains highly expressed in differentiated DCs. Transcription factors that are required to maintain the identity of terminally differentiated cells are designated "terminal selectors." Using BM chimeras, conditional Irf8(fl/fl) mice and various promotors to target Cre recombinase to different stages of monocyte and DC development, we have identified IRF8 as a terminal selector of the cDC1 lineage controlling survival. In monocytes, IRF8 was necessary during early but not late development. Complete or late deletion of IRF8 had no effect on pDC development or survival but altered their phenotype and gene-expression profile leading to increased T cell stimulatory function but decreased type 1 interferon production. Thus, IRF8 differentially controls the survival and function of terminally differentiated monocytes, cDC1s, and pDCs.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Transcription Factors / Dendritic Cells / Cell Differentiation / Interferon Regulatory Factors Type of study: Prognostic_studies Limits: Animals Language: En Journal: Immunity Journal subject: ALERGIA E IMUNOLOGIA Year: 2016 Type: Article Affiliation country: Belgium
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Transcription Factors / Dendritic Cells / Cell Differentiation / Interferon Regulatory Factors Type of study: Prognostic_studies Limits: Animals Language: En Journal: Immunity Journal subject: ALERGIA E IMUNOLOGIA Year: 2016 Type: Article Affiliation country: Belgium