Structural changes of Arthrospira sp. after low energy sonication treatment for microalgae harvesting: Elucidating key parameters to detect the rupture of gas vesicles.
Bioresour Technol
; 223: 98-104, 2017 Jan.
Article
in En
| MEDLINE
| ID: mdl-27788433
ABSTRACT
The buoyancy suppression by low energy sonication (LES) treatment (0.8W·mL-1, 20kHz, 10s) has recently been proposed as an initial harvesting step for Arthrospira sp. This paper aims to describe the structural changes in Arthrospira sp. after LES treatment and to present how these structural changes affect the results obtained by different analytical techniques. Transmission electron microscopy (TEM) micrographs of trichomes evidenced the gas vesicles rupture but also revealed a rearrangement of thylakoids and more visible phycobilisomes were observed. Differences between treated and untreated samples were detected by confocal microscopy, flow cytometry and optical microscopy but not by electrical impedance spectroscopy (EIS). After LES treatment, 2-fold increase in autofluorescence at 610/660nm was measured (phycocyanin/allophycocyanin emission wavelengths) and a ten-fold decrease in side scatter light intensity (due to a reduction of trichome's inner complexity). This was further confirmed by optical microscopy showing changes on trichomes appearance (from wrinkled to smooth).
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Sonication
/
Spirulina
/
Microalgae
Language:
En
Journal:
Bioresour Technol
Journal subject:
ENGENHARIA BIOMEDICA
Year:
2017
Type:
Article