ß-eudesmol suppresses allergic reactions via inhibiting mast cell degranulation.
Clin Exp Pharmacol Physiol
; 44(2): 257-265, 2017 02.
Article
in En
| MEDLINE
| ID: mdl-27862161
ABSTRACT
The regulatory effect of ß-eudesmol, which is an active constituent of Pyeongwee-San (KMP6), is evaluated for allergic reactions induced by mast cell degranulation. Phorbol 12-myristate 13-acetate (PMA) plus calcium ionophore A23187-stimulated human mast cell line, HMC-1 cells, and compound 48/80-stimulated rat peritoneal mast cells (RPMCs) are used as the in vitro models; mice models of systemic anaphylaxis, ear swelling, and IgE-dependent passive cutaneous anaphylaxis (PCA) are used as the in vivo allergic models. The results demonstrate that ß-eudesmol suppressed the histamine and tryptase releases from the PMA plus calcium ionophore A23187-stimulated HMC-1 cells. ß-eudesmol inhibits the expression and activity of histidine decarboxylase in the activated HMC-1 cells. In addition, ß-eudesmol inhibits the levels of histamine and tryptase released from the compound 48/80-stimulated RPMCs. Furthermore, ß-eudesmol decreases the intracellular calcium level in the activated RPMCs. ß-eudesmol also decreases the compound 48/80-induced mortality and ear swelling response. ß-eudesmol suppresses the serum levels of histamine, IgE, interleukin (IL)-1ß, IL-4, IL-5, IL-6, IL-13, and vascular endothelial growth factor (VEGF) under PCA mice as well as PCA reactions. Therefore, the results from this study indicate the potential of ß-eudesmol as an anti-allergic drug with respect to its pharmacological properties against mast cell-mediated allergic reactions.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Passive Cutaneous Anaphylaxis
/
Cell Degranulation
/
Anti-Allergic Agents
/
Sesquiterpenes, Eudesmane
/
Anaphylaxis
/
Mast Cells
Type of study:
Prognostic_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
Clin Exp Pharmacol Physiol
Year:
2017
Type:
Article