IgG anti-IgE from atopic dermatitis induces mediator release from basophils and mast cells.
J Invest Dermatol
; 93(2): 246-52, 1989 Aug.
Article
in En
| MEDLINE
| ID: mdl-2787823
ABSTRACT
IgG antibodies containing anti-IgE activity isolated from a patient with atopic dermatitis (H-aIgE) induced mediator release from human basophils and mast cells isolated from skin and lung tissues. The release of histamine was calcium- and temperature-dependent and did not involve cytotoxicity. There was an excellent correlation (r = 0.88; p less than 0.001) between the maximum percent histamine release from human basophils induced by rabbit anti-IgE (R-aIgE) and H-aIgE. H-aIgE was approximately 30 times more potent than R-aIgE in inducing mediator release from human basophils, skin, and lung mast cells. H-aIgE specifically desensitized human basophils to a subsequent challenge with both H-aIgE and R-aIgE. Lactic acid removal of IgE from human basophils blocked the releasing activity of both R-aIgE and H-aIgE. Passive sensitization with hyperimmune sera or purified IgE myeloma restored the response of basophils to both R-aIgE and H-aIgE. IgE purified from three different myeloma patients concentration-dependently blocked the histamine releasing activity of both R-aIgE and H-aIgE.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Basophils
/
Immunoglobulin E
/
Immunoglobulin G
/
Antibodies, Anti-Idiotypic
/
Dermatitis, Atopic
/
Mast Cells
Limits:
Adolescent
/
Child
/
Child, preschool
/
Humans
Language:
En
Journal:
J Invest Dermatol
Year:
1989
Type:
Article
Affiliation country:
Italy