Multilocus sequence analysis of Giardia spp. isolated from patients with diarrhea in Austria.
Parasitol Res
; 116(2): 477-481, 2017 Feb.
Article
in En
| MEDLINE
| ID: mdl-27885465
ABSTRACT
Giardia duodenalis is a protozoan parasite causing intestinal infections in a wide range of mammals. Two distinct assemblages, A and B, infect humans predominantly; however, both are believed to be generally zoonotic. Giardia strains associated with infections in Austria have not been investigated at the molecular level. In this study, 65 human stool samples microscopically positive for Giardia spp. were subjected to DNA isolation and nested PCR targeting fragments of the glutamate dehydrogenase (gdh), triose phosphate isomerase (tpi), and beta-gardin (bg) genes. A total of 52 samples were successfully analyzed using PCR and DNA sequencing. Assemblage B was detected most frequently and accounted for 65.4% (34/52) of infections, while Assemblage A accounted for 34.6% (18/52). There was a high level of genetic diversity among the isolates with 46.2% designated as sub-assemblage BIV (24/52), 25% sub-assemblage AII (13/52), 19.2% sub-assemblage BIII (10/52), and 9.6% sub-assemblage AI (5/52). No mixed infections were detected. The results suggest that the majority of infections were imported and that endemic anthroponotic transmission plays a minor role in Austria.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Giardiasis
/
Diarrhea
/
Giardia
Limits:
Animals
/
Humans
Country/Region as subject:
Europa
Language:
En
Journal:
Parasitol Res
Journal subject:
PARASITOLOGIA
Year:
2017
Type:
Article
Affiliation country:
Austria