Methods for the analysis of early oogenesis in Zebrafish.

Elkouby, Yaniv M; Mullins, Mary C

Elkouby, Yaniv M; Mullins, Mary C.

Affiliation

Elkouby YM; Department of Cell and Developmental Biology, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA.
Mullins MC; Department of Cell and Developmental Biology, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA.

Dev Biol ; 430(2): 310-324, 2017 10 15.

Article in En | MEDLINE | ID: mdl-27988227

Subject(s)

Oogenesis; Ovum/physiology; Specimen Handling/methods; Time-Lapse Imaging/methods; Animals; Cells, Cultured; Cytoskeleton/ultrastructure; DNA/analysis; Female; Genes, Reporter; In Situ Hybridization, Fluorescence/methods; Luminescent Proteins/analysis; Luminescent Proteins/genetics; Meiosis; Microscopy, Confocal/instrumentation; Microscopy, Confocal/methods; Oocytes/physiology; Oocytes/ultrastructure; Organelles/physiology; Organelles/ultrastructure; Ovary/cytology; Ovary/growth & development; Ovum/chemistry; Ovum/ultrastructure; RNA, Messenger/analysis; Sex Determination Processes; Staining and Labeling/methods; Zebrafish

Key words

Animal-vegetal axis; Balbiani body; Centrosome; Chromosomal bouquet; Immune-fluorescence; In situ hybridization; Live time-lapse imaging; Meiosis; Oocyte isolation; Oocyte polarity; Oogenesis; Ovarian culture; Ovary development; Quantitative image analysis; Symmetry-breaking; Zebrafish

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Oogenesis / Ovum / Specimen Handling / Time-Lapse Imaging Type of study: Prognostic_studies Limits: Animals Language: En Journal: Dev Biol Year: 2017 Type: Article Affiliation country: United States

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