Bioreaction Engineering Leading to Efficient Synthesis of L-Glyceraldehyd-3-Phosphate.
Biotechnol J
; 12(3)2017 Mar.
Article
in En
| MEDLINE
| ID: mdl-27992107
Enantiopure L-glyceraldehyde-3-phosphate (L-GAP) is a useful building block in natural biological and synthetic processes. A biocatalytic process using glycerol kinase from Cellulomonas sp. (EC 2.7.1.30) catalyzed phosphorylation of L-glyceraldehyde (L-GA) by ATP is used for the synthesis of L-GAP. L-GAP has a half-life of 6.86 h under reaction conditions. The activity of this enzyme depends on the Mg2+ to ATP molar ratio showing maximum activity at the optimum molar ratio of 0.7. A kinetic model is developed and validated showing a 2D correlation of 99.9% between experimental and numerical data matrices. The enzyme exhibits inhibition by ADP, AMP, methylglyoxal and Ca2+ , but not by L-GAP and inorganic orthophosphate. Moreover, equal amount of Ca2+ exerts a different degree of inhibition relative to the activity without the addition of Ca2+ depending on the Mg2+ to ATP molar ratio. If the Mg2+ to ATP molar ratio is set to be at the optimum value or less, inorganic hexametaphosphate (PPi6) suppresses the enzyme activity; otherwise PPi6 enhances the enzyme activity. Based on reaction engineering parameters such as conversion, selectivity and specific productivity, evaluation of different reactor types reveals that batchwise operation via stirred-tank reactor is the most efficient process for the synthesis of L-GAP.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Bioengineering
/
Glyceraldehyde 3-Phosphate
/
Glycerol Kinase
Type of study:
Prognostic_studies
Language:
En
Journal:
Biotechnol J
Journal subject:
BIOTECNOLOGIA
Year:
2017
Type:
Article
Affiliation country:
Germany