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Bioreaction Engineering Leading to Efficient Synthesis of L-Glyceraldehyd-3-Phosphate.
Molla, Getachew S; Kinfu, Birhanu M; Chow, Jennifer; Streit, Wolfgang; Wohlgemuth, Roland; Liese, Andreas.
Affiliation
  • Molla GS; Institute of Technical Biocatalysis, Hamburg University of Technology, Hamburg, Germany.
  • Kinfu BM; Department of Microbiology and Biotechnology, University of Hamburg, Hamburg, Germany.
  • Chow J; Department of Microbiology and Biotechnology, University of Hamburg, Hamburg, Germany.
  • Streit W; Department of Microbiology and Biotechnology, University of Hamburg, Hamburg, Germany.
  • Wohlgemuth R; Sigma-Aldrich Chemie GmbH, Member of Merck Group, Buchs, Switzerland.
  • Liese A; Institute of Technical Biocatalysis, Hamburg University of Technology, Hamburg, Germany.
Biotechnol J ; 12(3)2017 Mar.
Article in En | MEDLINE | ID: mdl-27992107
Enantiopure L-glyceraldehyde-3-phosphate (L-GAP) is a useful building block in natural biological and synthetic processes. A biocatalytic process using glycerol kinase from Cellulomonas sp. (EC 2.7.1.30) catalyzed phosphorylation of L-glyceraldehyde (L-GA) by ATP is used for the synthesis of L-GAP. L-GAP has a half-life of 6.86 h under reaction conditions. The activity of this enzyme depends on the Mg2+ to ATP molar ratio showing maximum activity at the optimum molar ratio of 0.7. A kinetic model is developed and validated showing a 2D correlation of 99.9% between experimental and numerical data matrices. The enzyme exhibits inhibition by ADP, AMP, methylglyoxal and Ca2+ , but not by L-GAP and inorganic orthophosphate. Moreover, equal amount of Ca2+ exerts a different degree of inhibition relative to the activity without the addition of Ca2+ depending on the Mg2+ to ATP molar ratio. If the Mg2+ to ATP molar ratio is set to be at the optimum value or less, inorganic hexametaphosphate (PPi6) suppresses the enzyme activity; otherwise PPi6 enhances the enzyme activity. Based on reaction engineering parameters such as conversion, selectivity and specific productivity, evaluation of different reactor types reveals that batchwise operation via stirred-tank reactor is the most efficient process for the synthesis of L-GAP.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bioengineering / Glyceraldehyde 3-Phosphate / Glycerol Kinase Type of study: Prognostic_studies Language: En Journal: Biotechnol J Journal subject: BIOTECNOLOGIA Year: 2017 Type: Article Affiliation country: Germany

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bioengineering / Glyceraldehyde 3-Phosphate / Glycerol Kinase Type of study: Prognostic_studies Language: En Journal: Biotechnol J Journal subject: BIOTECNOLOGIA Year: 2017 Type: Article Affiliation country: Germany