Centrifugal micro-channel array droplet generation for highly parallel digital PCR.
Lab Chip
; 17(2): 235-240, 2017 01 17.
Article
in En
| MEDLINE
| ID: mdl-28009866
ABSTRACT
Stable water-in-oil emulsion is essential to digital PCR and many other bioanalytical reactions that employ droplets as microreactors. We developed a novel technology to produce monodisperse emulsion droplets with high efficiency and high throughput using a bench-top centrifuge. Upon centrifugal spinning, the continuous aqueous phase is dispersed into monodisperse droplet jets in air through a micro-channel array (MiCA) and then submerged into oil as a stable emulsion. We performed dPCR reactions with a high dynamic range through the MiCA approach, and demonstrated that this cost-effective method not only eliminates the usage of complex microfluidic devices and control systems, but also greatly suppresses the loss of materials and cross-contamination. MiCA-enabled highly parallel emulsion generation combines both easiness and robustness of picoliter droplet production, and breaks the technical challenges by using conventional lab equipment and supplies.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Centrifugation
/
Polymerase Chain Reaction
/
Lab-On-A-Chip Devices
Language:
En
Journal:
Lab Chip
Journal subject:
BIOTECNOLOGIA
/
QUIMICA
Year:
2017
Type:
Article
Affiliation country:
China