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Coordinating cell cycle-regulated histone gene expression through assembly and function of the Histone Locus Body.
Duronio, Robert J; Marzluff, William F.
Affiliation
  • Duronio RJ; a Department of Biology , University of North Carolina , Chapel Hill , NC , USA.
  • Marzluff WF; b Department of Genetics , University of North Carolina , Chapel Hill , NC , USA.
RNA Biol ; 14(6): 726-738, 2017 06 03.
Article in En | MEDLINE | ID: mdl-28059623
ABSTRACT
Metazoan replication-dependent (RD) histone genes encode the only known cellular mRNAs that are not polyadenylated. These mRNAs end instead in a conserved stem-loop, which is formed by an endonucleolytic cleavage of the pre-mRNA. The genes for all 5 histone proteins are clustered in all metazoans and coordinately regulated with high levels of expression during S phase. Production of histone mRNAs occurs in a nuclear body called the Histone Locus Body (HLB), a subdomain of the nucleus defined by a concentration of factors necessary for histone gene transcription and pre-mRNA processing. These factors include the scaffolding protein NPAT, essential for histone gene transcription, and FLASH and U7 snRNP, both essential for histone pre-mRNA processing. Histone gene expression is activated by Cyclin E/Cdk2-mediated phosphorylation of NPAT at the G1-S transition. The concentration of factors within the HLB couples transcription with pre-mRNA processing, enhancing the efficiency of histone mRNA biosynthesis.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Histones / Cell Cycle / Gene Expression Regulation / Genetic Loci Limits: Animals / Humans Language: En Journal: RNA Biol Journal subject: BIOLOGIA MOLECULAR Year: 2017 Type: Article Affiliation country: United States

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Histones / Cell Cycle / Gene Expression Regulation / Genetic Loci Limits: Animals / Humans Language: En Journal: RNA Biol Journal subject: BIOLOGIA MOLECULAR Year: 2017 Type: Article Affiliation country: United States