Quantitation of rat cerebral blood flow using 99mTc-HMPAO.
Nucl Med Biol
; 47: 19-22, 2017 Apr.
Article
in En
| MEDLINE
| ID: mdl-28063322
ABSTRACT
INTRODUCTION:
Technetium-99m-hexamethylpropyleneamine oxime (99mTc-HMPAO) is potentially useful for the assessment of cerebral blood flow (CBF) in small animals. In this paper, a procedure for quantitation of rat CBF using 99mTc-HMPAO was determined.METHODS:
Biodistribution of 99mTc-radioactivity in normal rats was determined after intravenous administration of 99mTc-HMPAO. Acetazolamide treated rats were intravenously administered with the mixture of 99mTc-HMPAO and N-isopropyl-[125I]iodoamphetamine ([125I]IMP), and arterial blood was then collected for 5min. After blood sampling, the brain radioactivity concentration was measured with the auto-well γ counter.RESULTS:
The brain radioactivity concentration after intravenous administration of 99mTc-HMPAO was steady from 14s to 60min post-injection. A double tracer experiment using 99mTc-HMPAO and [125I]IMP showed that 19s was the average of the optimal integration interval of arterial blood 99mTc-radioactivity concentration to obtain CBF values measured by 99mTc-HMPAO identical to those determined by [125I]IMP. The CBF value determined by 99mTc-HMPAO, calculated by dividing the brain radioactivity concentration at 5min post-injection by the integrated arterial blood radioactivity concentration until 19s post-injection, was well correlated with CBF as determined by [125I]IMP.CONCLUSION:
These results suggest that the CBF quantitation procedure described in this paper could be useful for rat CBF assessment.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cerebrovascular Circulation
/
Technetium Tc 99m Exametazime
Limits:
Animals
Language:
En
Journal:
Nucl Med Biol
Journal subject:
BIOLOGIA
/
MEDICINA NUCLEAR
Year:
2017
Type:
Article
Affiliation country:
Japan