Structure/activity relationship of thapsigargin inhibition on the purified Golgi/secretory pathway Ca<sup>2+</sup>/Mn<sup>2+</sup>-transport ATPase (SPCA1a).

Chen, Jialin; De Raeymaecker, Joren; Hovgaard, Jannik Brøndsted; Smaardijk, Susanne; Vandecaetsbeek, Ilse; Wuytack, Frank; Møller, Jesper Vuust; Eggermont, Jan; De Maeyer, Marc; Christensen, Søren Brøgger; Vangheluwe, Peter

Structure/activity relationship of thapsigargin inhibition on the purified Golgi/secretory pathway Ca²⁺/Mn²⁺-transport ATPase (SPCA1a).

Chen, Jialin; De Raeymaecker, Joren; Hovgaard, Jannik Brøndsted; Smaardijk, Susanne; Vandecaetsbeek, Ilse; Wuytack, Frank; Møller, Jesper Vuust; Eggermont, Jan; De Maeyer, Marc; Christensen, Søren Brøgger; Vangheluwe, Peter.

Affiliation

Chen J; From the Laboratory of Cellular Transport Systems, Department of Cellular and Molecular Medicine, and.
De Raeymaecker J; Biochemistry, Molecular and Structural Biology Section, Department of Chemistry, KU Leuven, 3000 Leuven, Belgium.
Hovgaard JB; the Department of Drug Design and Pharmacology, University of Copenhagen, DK-2100 Copenhagen, Denmark, and.
Smaardijk S; From the Laboratory of Cellular Transport Systems, Department of Cellular and Molecular Medicine, and.
Vandecaetsbeek I; From the Laboratory of Cellular Transport Systems, Department of Cellular and Molecular Medicine, and.
Wuytack F; From the Laboratory of Cellular Transport Systems, Department of Cellular and Molecular Medicine, and.
Møller JV; the Department of Biomedicine, University of Aarhus, 8000 Aarhus, Denmark.
Eggermont J; From the Laboratory of Cellular Transport Systems, Department of Cellular and Molecular Medicine, and.
De Maeyer M; Biochemistry, Molecular and Structural Biology Section, Department of Chemistry, KU Leuven, 3000 Leuven, Belgium.
Christensen SB; the Department of Drug Design and Pharmacology, University of Copenhagen, DK-2100 Copenhagen, Denmark, and.
Vangheluwe P; From the Laboratory of Cellular Transport Systems, Department of Cellular and Molecular Medicine, and peter.vangheluwe@kuleuven.be.

J Biol Chem ; 292(17): 6938-6951, 2017 04 28.

Article in En | MEDLINE | ID: mdl-28264934

Subject(s)

Calcium-Transporting ATPases/antagonists & inhibitors; Calcium-Transporting ATPases/chemistry; Sarcoplasmic Reticulum Calcium-Transporting ATPases/antagonists & inhibitors; Thapsigargin/chemistry; Animals; Antineoplastic Agents/therapeutic use; Breast Neoplasms/drug therapy; Calcium/chemistry; Cholesterol/chemistry; Drug Design; Female; Humans; Hydroquinones/chemistry; Indoles/chemistry; Linoleic Acids/chemistry; Liposomes/chemistry; Male; Mutagenesis, Site-Directed; Oleic Acids/chemistry; Phosphatidic Acids/chemistry; Prostatic Neoplasms/drug therapy; Protein Binding; Protein Conformation; Rabbits; Recombinant Proteins/chemistry; Saccharomyces cerevisiae/metabolism; Sarcoplasmic Reticulum Calcium-Transporting ATPases/chemistry; Sphingomyelins/chemistry; Structure-Activity Relationship

Key words

Golgi; calcium transport; cholesterol; cyclopiazonic acid; membrane transporter reconstitution; molecular modeling; prostate cancer; protein purification; thapsigargin

Fulltext

XML

PubMed Links

Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Calcium-Transporting ATPases / Thapsigargin / Sarcoplasmic Reticulum Calcium-Transporting ATPases Type of study: Prognostic_studies Limits: Animals / Female / Humans / Male Language: En Journal: J Biol Chem Year: 2017 Type: Article

Fulltext

XML

PubMed Links

Search on Google