Development of induced glioblastoma by implantation of a human xenograft in Yucatan minipig as a large animal model.
J Neurosci Methods
; 282: 61-68, 2017 Apr 15.
Article
in En
| MEDLINE
| ID: mdl-28284687
ABSTRACT
BACKGROUND:
Glioblastoma is the most common and deadliest primary brain tumor for humans. Despite many efforts toward the improvement of therapeutic methods, prognosis is poor and the disease remains incurable with a median survival of 12-14.5 months after an optimal treatment. To develop novel treatment modalities for this fatal disease, new devices must be tested on an ideal animal model before performing clinical trials in humans. NEWMETHOD:
A new model of induced glioblastoma in Yucatan minipigs was developed. Nine immunosuppressed minipigs were implanted with the U87 human glioblastoma cell line in both the left and right hemispheres. Computed tomography (CT) acquisitions were performed once a week to monitor tumor growth.RESULTS:
Among the 9 implanted animals, 8 minipigs showed significant macroscopic tumors on CT acquisitions. Histological examination of the brain after euthanasia confirmed the CT imaging findings with the presence of an undifferentiated glioma. COMPARISON WITH EXISTINGMETHOD:
Yucatan minipig, given its brain size and anatomy (gyrencephalic structure) which are comparable to humans, provides a reliable brain tumor model for preclinical studies of different therapeuticMETHODS:
in realistic conditions. Moreover, the short development time, the lower cyclosporine and caring cost and the compatibility with the size of commercialized stereotactic frames make it an affordable and practical animal model, especially in comparison with large breed pigs.CONCLUSION:
This reproducible glioma model could simulate human anatomical conditions in preclinical studies and facilitate the improvement of novel therapeutic devices, designed at the human scale from the outset.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Swine, Miniature
/
Brain Neoplasms
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Glioblastoma
/
Neoplasm Transplantation
Type of study:
Prognostic_studies
Limits:
Animals
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Female
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Humans
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Male
Language:
En
Journal:
J Neurosci Methods
Year:
2017
Type:
Article