Entrapment of DyP-type peroxidase from Pseudomonas fluorescens Pf-5 into Ca-alginate magnetic beads.
Biotechnol Appl Biochem
; 65(2): 238-245, 2018 Mar.
Article
in En
| MEDLINE
| ID: mdl-28326617
ABSTRACT
The aim of this study was to investigate the optimal conditions for the immobilization and stabilization of DyP1B dye decolorizing peroxidases type B (DyP1B) from Pseudomonas fluorescens Pf-5 immobilized in Ca-alginate ferromagnetic beads. The immobilized DyP1B was used in the degradation of the Reactive Blue 5 (RB5) synthetic dye. The enzyme was successfully entrapped in a Ca-alginate matrix and showed an encapsulation efficiency of 94%. The concentration of DyP1B (0.8 mg mL-1 ), 2% of alginate and magnetite (10.0 mg mL-1 ) was optimal for immobilization. The immobilized DyP1B showed optimum activity at pH 7.0 and 40 °C compared with pH 5.5 and 30 °C for free peroxidase. Reusability studies showed that after five cycles, the immobilized DyP1B system retained more than 58% of its initial activity. The immobilized DyP1B was able to decolorize RB5 at concentrations of 0.1, 0.05, and 0.01% (w v-1 ) with efficiency rates of about 20, 29, and 45%, respectively. The immobilization of DyP1B in alginate beads with the addition of Fe3 O4 increased its catalytic and applicative potential.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pseudomonas fluorescens
/
Anthraquinones
/
Peroxidase
/
Coloring Agents
/
Alginates
/
Enzymes, Immobilized
/
Magnets
Language:
En
Journal:
Biotechnol Appl Biochem
Journal subject:
BIOQUIMICA
/
BIOTECNOLOGIA
Year:
2018
Type:
Article
Affiliation country:
Poland