Co-cultivation of Chlamydomonas reinhardtii with Azotobacter chroococcum improved H2 production.
Biotechnol Lett
; 39(5): 731-738, 2017 May.
Article
in En
| MEDLINE
| ID: mdl-28432498
ABSTRACT
OBJECTIVES:
To improve H2 production, the green algae Chlamydomonas reinhardtii cc849 was co-cultured with Azotobacter chroococcum.RESULTS:
The maximum H2 production of the co-culture was 350% greater than that of the pure algal cultures under optimal H2 production conditions. The maximum growth and the respiratory rate of the co-cultures were about 320 and 300% of the controls, and the dissolved O2 of co-cultures was decreased 74%. Furthermore, the in vitro maximum hydrogenase activity of the co-culture was 250% greater than that of the control, and the in vivo maximum hydrogenase activity of the co-culture was 1.4-fold greater than that of the control. In addition, the maximum starch content of co-culture was 1400% that of the control.CONCLUSIONS:
Azotobacter chroococcum improved the H2 production of the co-cultures by decreasing the O2 content and increasing the growth and starch content of the algae and the hydrogenase activity of the co-cultures relative to those of pure algal cultures.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Azotobacter
/
Chlamydomonas reinhardtii
/
Coculture Techniques
/
Bioreactors
/
Hydrogen
Language:
En
Journal:
Biotechnol Lett
Year:
2017
Type:
Article