A single L288I substitution in the fusion protein of bovine parainfluenza virus type 3 enhances virus growth in semi-suitable cell lines.
Arch Virol
; 162(8): 2409-2413, 2017 Aug.
Article
in En
| MEDLINE
| ID: mdl-28451903
ABSTRACT
The bovine parainfluenza virus type 3 BN-CE vaccine strain was obtained by serial passage of the BN-1 strain in chicken embryonic fibroblasts (CEF). We previously identified a substitution (L288I) in the fusion (F) protein between the two strains. To examine the effect of the substitution on CEF adaptation and attenuation, we generated a recombinant BN-1 strain with the L288I substitution in the F protein (FL288I-EGFP). FL288I-EGFP replicated more efficiently than a recombinant BN-1 strain (wt-EGFP) in semi-suitable cell lines, suggesting that the L288I substitution was established in the BN-1 strain during the process of adaptation in CEF.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Adaptation, Physiological
/
Viral Fusion Proteins
/
Amino Acid Substitution
/
Parainfluenza Virus 3, Bovine
Limits:
Animals
/
Humans
Language:
En
Journal:
Arch Virol
Year:
2017
Type:
Article
Affiliation country:
Japan