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Mechanism and color modulation of fungal bioluminescence.
Kaskova, Zinaida M; Dörr, Felipe A; Petushkov, Valentin N; Purtov, Konstantin V; Tsarkova, Aleksandra S; Rodionova, Natalja S; Mineev, Konstantin S; Guglya, Elena B; Kotlobay, Alexey; Baleeva, Nadezhda S; Baranov, Mikhail S; Arseniev, Alexander S; Gitelson, Josef I; Lukyanov, Sergey; Suzuki, Yoshiki; Kanie, Shusei; Pinto, Ernani; Di Mascio, Paolo; Waldenmaier, Hans E; Pereira, Tatiana A; Carvalho, Rodrigo P; Oliveira, Anderson G; Oba, Yuichi; Bastos, Erick L; Stevani, Cassius V; Yampolsky, Ilia V.
Affiliation
  • Kaskova ZM; Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, Moscow 117997, Russia.
  • Dörr FA; Pirogov Russian National Research Medical University, Ostrovitianov 1, Moscow 117997, Russia.
  • Petushkov VN; Institute of Biophysics Siberian Branch of Russian Academy of Sciences (SB RAS), Federal Research Center "Krasnoyarsk Science Center SB RAS," Akademgorodok, Krasnoyarsk 660036, Russia.
  • Purtov KV; Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo, 05508-900, Brazil.
  • Tsarkova AS; Institute of Biophysics Siberian Branch of Russian Academy of Sciences (SB RAS), Federal Research Center "Krasnoyarsk Science Center SB RAS," Akademgorodok, Krasnoyarsk 660036, Russia.
  • Rodionova NS; Institute of Biophysics Siberian Branch of Russian Academy of Sciences (SB RAS), Federal Research Center "Krasnoyarsk Science Center SB RAS," Akademgorodok, Krasnoyarsk 660036, Russia.
  • Mineev KS; Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, Moscow 117997, Russia.
  • Guglya EB; Pirogov Russian National Research Medical University, Ostrovitianov 1, Moscow 117997, Russia.
  • Kotlobay A; Institute of Biophysics Siberian Branch of Russian Academy of Sciences (SB RAS), Federal Research Center "Krasnoyarsk Science Center SB RAS," Akademgorodok, Krasnoyarsk 660036, Russia.
  • Baleeva NS; Institute of Biophysics Siberian Branch of Russian Academy of Sciences (SB RAS), Federal Research Center "Krasnoyarsk Science Center SB RAS," Akademgorodok, Krasnoyarsk 660036, Russia.
  • Baranov MS; Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, Moscow 117997, Russia.
  • Arseniev AS; Pirogov Russian National Research Medical University, Ostrovitianov 1, Moscow 117997, Russia.
  • Gitelson JI; Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, Moscow 117997, Russia.
  • Lukyanov S; Institute of Biophysics Siberian Branch of Russian Academy of Sciences (SB RAS), Federal Research Center "Krasnoyarsk Science Center SB RAS," Akademgorodok, Krasnoyarsk 660036, Russia.
  • Suzuki Y; Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, Moscow 117997, Russia.
  • Kanie S; Pirogov Russian National Research Medical University, Ostrovitianov 1, Moscow 117997, Russia.
  • Pinto E; Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, Moscow 117997, Russia.
  • Di Mascio P; Pirogov Russian National Research Medical University, Ostrovitianov 1, Moscow 117997, Russia.
  • Waldenmaier HE; Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, Moscow 117997, Russia.
  • Pereira TA; Institute of Biophysics Siberian Branch of Russian Academy of Sciences (SB RAS), Federal Research Center "Krasnoyarsk Science Center SB RAS," Akademgorodok, Krasnoyarsk 660036, Russia.
  • Carvalho RP; Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, Moscow 117997, Russia.
  • Oliveira AG; Pirogov Russian National Research Medical University, Ostrovitianov 1, Moscow 117997, Russia.
  • Oba Y; Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan.
  • Bastos EL; Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan.
  • Stevani CV; Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo, 05508-900, Brazil.
  • Yampolsky IV; Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, 05508-000, Brazil.
Sci Adv ; 3(4): e1602847, 2017 Apr.
Article in En | MEDLINE | ID: mdl-28508049
ABSTRACT
Bioluminescent fungi are spread throughout the globe, but details on their mechanism of light emission are still scarce. Usually, the process involves three key components an oxidizable luciferin substrate, a luciferase enzyme, and a light emitter, typically oxidized luciferin, and called oxyluciferin. We report the structure of fungal oxyluciferin, investigate the mechanism of fungal bioluminescence, and describe the use of simple synthetic α-pyrones as luciferins to produce multicolor enzymatic chemiluminescence. A high-energy endoperoxide is proposed as an intermediate of the oxidation of the native luciferin to the oxyluciferin, which is a pyruvic acid adduct of caffeic acid. Luciferase promiscuity allows the use of simple α-pyrones as chemiluminescent substrates.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Pyrazines / Fungal Proteins / Luminescence / Fungi / Indoles / Luciferases Language: En Journal: Sci Adv Year: 2017 Type: Article Affiliation country: RUSSIA
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Pyrazines / Fungal Proteins / Luminescence / Fungi / Indoles / Luciferases Language: En Journal: Sci Adv Year: 2017 Type: Article Affiliation country: RUSSIA