Glycine Substitution at Helix-to-Coil Transitions Facilitates the Structural Determination of a Stabilized Subtype C HIV Envelope Glycoprotein.

Guenaga, Javier; Garces, Fernando; de Val, Natalia; Stanfield, Robyn L; Dubrovskaya, Viktoriya; Higgins, Brett; Carrette, Barbara; Ward, Andrew B; Wilson, Ian A; Wyatt, Richard T

Guenaga, Javier; Garces, Fernando; de Val, Natalia; Stanfield, Robyn L; Dubrovskaya, Viktoriya; Higgins, Brett; Carrette, Barbara; Ward, Andrew B; Wilson, Ian A; Wyatt, Richard T.

Affiliation

Guenaga J; IAVI Neutralizing Antibody Center at The Scripps Research Institute, La Jolla, CA 92037, USA.
Garces F; Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
de Val N; Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
Stanfield RL; IAVI Neutralizing Antibody Center at The Scripps Research Institute, La Jolla, CA 92037, USA; Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
Dubrovskaya V; Department of Immunology and Microbiology, The Scripps Research Institute, La Jolla, CA 92037, USA.
Higgins B; Department of Immunology and Microbiology, The Scripps Research Institute, La Jolla, CA 92037, USA.
Carrette B; IAVI Neutralizing Antibody Center at The Scripps Research Institute, La Jolla, CA 92037, USA.
Ward AB; IAVI Neutralizing Antibody Center at The Scripps Research Institute, La Jolla, CA 92037, USA; Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA; Scripps Center for HIV/AIDS Vaccine Immunology & Immunogen Discovery (CHAVI-ID),
Wilson IA; IAVI Neutralizing Antibody Center at The Scripps Research Institute, La Jolla, CA 92037, USA; Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA; Scripps Center for HIV/AIDS Vaccine Immunology & Immunogen Discovery (CHAVI-ID),
Wyatt RT; IAVI Neutralizing Antibody Center at The Scripps Research Institute, La Jolla, CA 92037, USA; Department of Immunology and Microbiology, The Scripps Research Institute, La Jolla, CA 92037, USA; Scripps Center for HIV/AIDS Vaccine Immunology & Immunogen Discovery (CHAVI-ID), La Jolla, CA 92037, U

Immunity ; 46(5): 792-803.e3, 2017 05 16.

Article in En | MEDLINE | ID: mdl-28514686

Subject(s)

Amino Acid Substitution; Glycine/chemistry; HIV-1/immunology; Protein Conformation, alpha-Helical; env Gene Products, Human Immunodeficiency Virus/chemistry; env Gene Products, Human Immunodeficiency Virus/immunology; Antibodies, Neutralizing/chemistry; Antibodies, Neutralizing/immunology; Antibodies, Neutralizing/metabolism; Binding Sites; Genotype; Glycine/genetics; Glycosylation; HIV Antibodies/chemistry; HIV Antibodies/immunology; HIV Antibodies/metabolism; HIV Envelope Protein gp120/chemistry; HIV Envelope Protein gp120/genetics; HIV Envelope Protein gp120/immunology; HIV Envelope Protein gp41/chemistry; HIV Envelope Protein gp41/genetics; HIV Envelope Protein gp41/immunology; HIV-1/classification; HIV-1/genetics; Humans; Models, Molecular; Mutation; Protein Binding/immunology; Protein Engineering; Protein Multimerization; Protein Stability; Proteolysis; Solubility; Structure-Activity Relationship; env Gene Products, Human Immunodeficiency Virus/genetics

Key words

Antibody; Envelope glycoprotein; Glycan shield; HIV; Immunogen; Trimer; Vaccine; bNAb

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: HIV-1 / Amino Acid Substitution / Env Gene Products, Human Immunodeficiency Virus / Protein Conformation, alpha-Helical / Glycine Limits: Humans Language: En Journal: Immunity Journal subject: ALERGIA E IMUNOLOGIA Year: 2017 Type: Article Affiliation country: United States

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