Anti-inflammatory effects of a traditional Korean medicine: Ojayeonjonghwan.
Pharm Biol
; 55(1): 1856-1862, 2017 Dec.
Article
in En
| MEDLINE
| ID: mdl-28614972
ABSTRACT
OBJECTIVE:
To study the anti-inflammatory properties of OJ. CONTEXT Ojayeonjonghwan (OJ) is a traditional Korean prescription, which has been widely used for the treatment of prostatitis. However, no scientific study has been performed of the anti-inflammatory effects of OJ. MATERIALS ANDMETHODS:
Peritoneal macrophages were isolated 3-4 days after injecting a C57BL/6J mouse with thioglycollate. They were then treated with OJ water extract (0.01, 0.1, and 1 mg/mL) for 1 h and stimulated with lipopolysaccharide (LPS) for different times. Nitric oxide (NO), inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2, and proinflammatory cytokine levels were determined by NO assay, Western blotting, RT-PCR and ELISA.RESULTS:
NO generation and iNOS induction were increased in the LPS-activated mouse peritoneal macrophages. However, NO generation and iNOS induction by LPS were suppressed by treatment with OJ for the first time. The IC50 value of OJ with respect to NO production was 0.09 mg/mL. OJ did not influence LPS-stimulated COX-2 induction, but did significantly decrease LPS-stimulated secretions and mRNA expressions of tumour necrosis factor (TNF)-α, interleukin (IL)-6, and IL-1ß. Inhibition rates of TNF-α, IL-6, and IL-1ß at an OJ concentration of 1 mg/mL were 77%, 88%, and 50%, respectively. OJ also suppressed the LPS-induced nuclear translocation of NF-κB. High-performance liquid chromatography showed schizandrin and gomisin A are major components of OJ.CONCLUSIONS:
OJ reduces inflammatory response, and this probably explains its positive impact on the prostatitis associated inflammation.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Polycyclic Compounds
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Plant Extracts
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Anti-Inflammatory Agents, Non-Steroidal
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Gene Expression Regulation
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Macrophages, Peritoneal
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Lignans
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Cyclooctanes
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Dioxoles
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Macrophage Activation
Limits:
Animals
Language:
En
Journal:
Pharm Biol
Year:
2017
Type:
Article