Actin turnover maintains actin filament homeostasis during cytokinetic ring contraction.
J Cell Biol
; 216(9): 2657-2667, 2017 09 04.
Article
in En
| MEDLINE
| ID: mdl-28655757
ABSTRACT
Cytokinesis in many eukaryotes involves a tension-generating actomyosin-based contractile ring. Many components of actomyosin rings turn over during contraction, although the significance of this turnover has remained enigmatic. Here, using Schizosaccharomyces japonicus, we investigate the role of turnover of actin and myosin II in its contraction. Actomyosin ring components self-organize into â¼1-µm-spaced clusters instead of undergoing full-ring contraction in the absence of continuous actin polymerization. This effect is reversed when actin filaments are stabilized. We tested the idea that the function of turnover is to ensure actin filament homeostasis in a synthetic system, in which we abolished turnover by fixing rings in cell ghosts with formaldehyde. We found that these rings contracted fully upon exogenous addition of a vertebrate myosin. We conclude that actin turnover is required to maintain actin filament homeostasis during ring contraction and that the requirement for turnover can be bypassed if homeostasis is achieved artificially.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Schizosaccharomyces
/
Actin Cytoskeleton
/
Actomyosin
/
Actins
/
Cytokinesis
Language:
En
Journal:
J Cell Biol
Year:
2017
Type:
Article
Affiliation country:
United kingdom