Identifying and Validating Tankyrase Binders and Substrates: A Candidate Approach.

Pollock, Katie; Ranes, Michael; Collins, Ian; Guettler, Sebastian

Pollock, Katie; Ranes, Michael; Collins, Ian; Guettler, Sebastian.

Affiliation

Pollock K; Division of Structural Biology, The Institute of Cancer Research, London, SW7 3RP, UK.
Ranes M; Division of Cancer Biology, The Institute of Cancer Research, London, SW7 3RP, UK.
Collins I; Division of Cancer Therapeutics, Cancer Research UK Cancer Therapeutics Unit, The Institute of Cancer Research, London, SW7 3RP, UK.
Guettler S; Division of Structural Biology, The Institute of Cancer Research, London, SW7 3RP, UK.

Methods Mol Biol ; 1608: 445-473, 2017.

Article in En | MEDLINE | ID: mdl-28695526

Subject(s)

Poly ADP Ribosylation/physiology; Tankyrases/chemistry; Tankyrases/metabolism; Animals; Binding Sites; Humans; Poly ADP Ribosylation/genetics; Protein Binding/genetics; Protein Binding/physiology; Telomere/genetics; Telomere/metabolism

Key words

Enzymesubstrate relationships; Fluorescence polarization (FP); PARP; Poly(ADP-ribosyl)ation; Protein expression; Protein purification; Protein-protein interactions; Structural biology; Tankyrase; Tankyrase-binding peptide motif

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Tankyrases / Poly ADP Ribosylation Limits: Animals / Humans Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2017 Type: Article Affiliation country: United kingdom

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