Production of Single Contracting Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes: Matrigel Mattress Technique.
Curr Protoc Stem Cell Biol
; 42: 4A.14.1-4A.14.7, 2017 Aug 14.
Article
in En
| MEDLINE
| ID: mdl-28806851
ABSTRACT
This unit describes the published Matrigel mattress method. Briefly, we describe the preparation of the mattress, replating of the human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) on the Matrigel mattress, and hiPSC-CM mattress maintenance. Adherence to this protocol will yield individual, robustly shortening hiPSC-CMs, which can be used for downstream applications. © 2017 by John Wiley & Sons, Inc.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Proteoglycans
/
Collagen
/
Laminin
/
Cell Culture Techniques
/
Myocytes, Cardiac
/
Induced Pluripotent Stem Cells
/
Myocardial Contraction
Limits:
Humans
Language:
En
Journal:
Curr Protoc Stem Cell Biol
Year:
2017
Type:
Article