Your browser doesn't support javascript.
loading
An improved ATAC-seq protocol reduces background and enables interrogation of frozen tissues.
Corces, M Ryan; Trevino, Alexandro E; Hamilton, Emily G; Greenside, Peyton G; Sinnott-Armstrong, Nicholas A; Vesuna, Sam; Satpathy, Ansuman T; Rubin, Adam J; Montine, Kathleen S; Wu, Beijing; Kathiria, Arwa; Cho, Seung Woo; Mumbach, Maxwell R; Carter, Ava C; Kasowski, Maya; Orloff, Lisa A; Risca, Viviana I; Kundaje, Anshul; Khavari, Paul A; Montine, Thomas J; Greenleaf, William J; Chang, Howard Y.
Affiliation
  • Corces MR; Center for Personal Dynamic Regulomes, Stanford University School of Medicine, Stanford, California, USA.
  • Trevino AE; Department of Dermatology, Stanford University School of Medicine, Stanford, California, USA.
  • Hamilton EG; Center for Personal Dynamic Regulomes, Stanford University School of Medicine, Stanford, California, USA.
  • Greenside PG; Department of Genetics, Stanford University School of Medicine, Stanford, California, USA.
  • Sinnott-Armstrong NA; Department of Bioengineering, Stanford University School of Medicine and School of Engineering, Stanford, California, USA.
  • Vesuna S; Program in Cancer Biology, Stanford University School of Medicine, Stanford, California, USA.
  • Satpathy AT; Department of Genetics, Stanford University School of Medicine, Stanford, California, USA.
  • Rubin AJ; Program in Biomedical Informatics, Stanford University School of Medicine, Stanford, California, USA.
  • Montine KS; Department of Genetics, Stanford University School of Medicine, Stanford, California, USA.
  • Wu B; Department of Bioengineering, Stanford University School of Medicine and School of Engineering, Stanford, California, USA.
  • Kathiria A; Center for Personal Dynamic Regulomes, Stanford University School of Medicine, Stanford, California, USA.
  • Cho SW; Department of Pathology, Stanford University School of Medicine, Stanford, California, USA.
  • Mumbach MR; Department of Dermatology, Stanford University School of Medicine, Stanford, California, USA.
  • Carter AC; Department of Pathology, Stanford University School of Medicine, Stanford, California, USA.
  • Kasowski M; Department of Genetics, Stanford University School of Medicine, Stanford, California, USA.
  • Orloff LA; Department of Genetics, Stanford University School of Medicine, Stanford, California, USA.
  • Risca VI; Center for Personal Dynamic Regulomes, Stanford University School of Medicine, Stanford, California, USA.
  • Kundaje A; Department of Dermatology, Stanford University School of Medicine, Stanford, California, USA.
  • Khavari PA; Center for Personal Dynamic Regulomes, Stanford University School of Medicine, Stanford, California, USA.
  • Montine TJ; Department of Genetics, Stanford University School of Medicine, Stanford, California, USA.
  • Greenleaf WJ; Center for Personal Dynamic Regulomes, Stanford University School of Medicine, Stanford, California, USA.
  • Chang HY; Department of Dermatology, Stanford University School of Medicine, Stanford, California, USA.
Nat Methods ; 14(10): 959-962, 2017 Oct.
Article in En | MEDLINE | ID: mdl-28846090
ABSTRACT
We present Omni-ATAC, an improved ATAC-seq protocol for chromatin accessibility profiling that works across multiple applications with substantial improvement of signal-to-background ratio and information content. The Omni-ATAC protocol generates chromatin accessibility profiles from archival frozen tissue samples and 50-µm sections, revealing the activities of disease-associated DNA elements in distinct human brain structures. The Omni-ATAC protocol enables the interrogation of personal regulomes in tissue context and translational studies.
Subject(s)
Fulltext
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Specimen Handling / DNA / Genome / Freezing Limits: Animals / Humans Language: En Journal: Nat Methods Journal subject: TECNICAS E PROCEDIMENTOS DE LABORATORIO Year: 2017 Type: Article Affiliation country: United States
Fulltext
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Specimen Handling / DNA / Genome / Freezing Limits: Animals / Humans Language: En Journal: Nat Methods Journal subject: TECNICAS E PROCEDIMENTOS DE LABORATORIO Year: 2017 Type: Article Affiliation country: United States