Iron and Electron Shuttle Mediated (Bio)degradation of 2,4-Dinitroanisole (DNAN).
Environ Sci Technol
; 51(18): 10729-10735, 2017 Sep 19.
Article
in En
| MEDLINE
| ID: mdl-28849653
ABSTRACT
The Department of Defense has developed explosives with the insensitive munition 2,4-dinitroanisole (DNAN), to prevent accidental detonations during training and operations. Understanding the fate and transport of DNAN is necessary to assess the risk it may represent to groundwater once the new ordnance is routinely produced and used. Experiments with ferrous iron or anthrahydroquinone-2,6-disulfonate (AH2QDS) were conducted from pH 6.0 to 9.0 with initial DNAN concentrations of 100 µM. DNAN was degraded by 1.2 mM Fe(II) at pH 7, 8, and 9, and rates increased with increasing pH. Greater than 90% of the initial 100 µM DNAN was reduced within 10 min at pH 9, and all DNAN was reduced within 1 h. AH2QDS reduced DNAN at all pH values tested. Cells of Geobacter metallireducens were added in the presence and absence of Fe(III) and/or anthraquinone-2,6-disulfonate (AQDS), and DNAN was also reduced in all cell suspensions. Cells reduced the compound directly, but both AQDS and Fe(III) increased the reaction rate, via the production of AH2QDS and/or Fe(II). DNAN was degraded via two intermediates 2-methoxy-5-nitroaniline and 4-methoxy-3-nitroaniline, to the amine product 2,4-diaminoanisole. These data suggest that an effective strategy can be developed for DNAN attenuation based on combined biological-abiotic reactions mediated by Fe(III)-reducing microorganisms.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Geobacter
/
Anisoles
Type of study:
Etiology_studies
/
Risk_factors_studies
Language:
En
Journal:
Environ Sci Technol
Year:
2017
Type:
Article
Affiliation country:
United States