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Multiple tissue methylation analysis of HTR2A exon I in suicidal behavior.
Bani-Fatemi, Ali; Strauss, John; Zai, Clement; Wong, Albert H C; de Luca, Vincenzo.
Affiliation
  • Bani-Fatemi A; aGroup for Suicide Studies, Centre for Addiction and Mental Health bInstitute of Medical Science cDepartment of Psychiatry, University of Toronto, Toronto, Ontario, Canada.
Psychiatr Genet ; 27(6): 219-224, 2017 12.
Article in En | MEDLINE | ID: mdl-29068870
ABSTRACT

OBJECTIVE:

The main aim of the current study was to investigate epigenetic alterations in serotonin 2A receptor (HTR2A) exon I CpG sites as possible risk factors for suicidal behavior. We also aimed to analyze the epigenetic alterations in two different tissues as epigenetic mechanisms are tissue specific. These epigenetic changes may lead to a better prediction of suicidal behavior.

METHODS:

Direct CpG methylation analysis was carried out on genomic DNA from the saliva of 20 schizophrenia suicide attempters and 27 non-attempters, and from post-mortem brain tissues of nine suicide victims and 11 controls. We used bisulfite pyrosequencing to assess the contributions of six CpG sites including the rs6313 (C102T) site in the first exon of HTR2A in suicide attempters and suicide victims.

RESULTS:

DNA methylation analysis did not find a significant difference in CpG methylation between suicide attempters and non-attempters (P=0.759) or between suicide victims and controls (P=0.189). We found a strong positive correlation between CpG methylation levels in blood and saliva (r=0.547, P<0.001).

DISCUSSION:

DNA methylation analysis confirmed that the overall methylation level of HTR2A exon I was around 80% for DNA extracted from saliva and almost 30% in the frontal cortex DNA. The results of this investigation do not support the evidence that methylation analysis of the HTR2A may be useful for investigating the epigenetic factors involved in suicidal behavior.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Suicide / Self-Injurious Behavior / DNA Methylation / Receptor, Serotonin, 5-HT2A Type of study: Prognostic_studies / Qualitative_research / Risk_factors_studies Limits: Adult / Humans / Male Language: En Journal: Psychiatr Genet Journal subject: GENETICA / PSIQUIATRIA Year: 2017 Type: Article Affiliation country: Canada

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Suicide / Self-Injurious Behavior / DNA Methylation / Receptor, Serotonin, 5-HT2A Type of study: Prognostic_studies / Qualitative_research / Risk_factors_studies Limits: Adult / Humans / Male Language: En Journal: Psychiatr Genet Journal subject: GENETICA / PSIQUIATRIA Year: 2017 Type: Article Affiliation country: Canada