Potential cow milk xanthine oxidase inhibitory and antioxidant activity of selected phenolic acid derivatives.
J Biochem Mol Toxicol
; 32(1)2018 Jan.
Article
in En
| MEDLINE
| ID: mdl-29071757
ABSTRACT
Xanthine oxidase (XO) found in all mammals and excess activity leads to urolithiasis. The cow milk XO was purified to 305-fold with a specific activity of 8.76 EU/mg and overall yield of 87% by using DEAE-Sepharose chromatography. The phenolics showed potent XO inhibitory effect with Ki , P1 (0.412), P2 (0.632), P3 (0.585), P4 (0.886), P5 (1.633), P6 (0.503), P7 (2.882), P8 (3.761), P9 (4.487), and P10 (5.841) µM. The phenolics P9 and P10 exhibited uncompetitive inhibition; the phenolics P1, P2, P3, P4, and P6 showed competitive inhibition, and other phenolic acids showed noncompetitive inhibition. The studied phenolic compounds showed potent antioxidant activity and expressed as EC50 , ranged from, DPPH (4.2-25.8 µg mL-1 ), ABTS (10.2-42.5 mmol TE 100 g-1 ), and FRAP (6.3-36.8 mol Fe (II) 100 g-1 ). The results obtained from this study might be utilized for design of XO inhibitors and as antigout agent.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Xanthine Oxidase
/
Cinnamates
/
Enzyme Inhibitors
/
Gallic Acid
/
Milk Proteins
/
Antioxidants
Limits:
Animals
Language:
En
Journal:
J Biochem Mol Toxicol
Journal subject:
BIOLOGIA MOLECULAR
/
BIOQUIMICA
/
TOXICOLOGIA
Year:
2018
Type:
Article