Characterization of Trichococcus paludicola sp. nov. and Trichococcus alkaliphilus sp. nov., isolated from a high-elevation wetland, by phenotypic and genomic analyses.
Int J Syst Evol Microbiol
; 68(1): 99-105, 2018 Jan.
Article
in En
| MEDLINE
| ID: mdl-29116035
ABSTRACT
Two psychrotolerant facultative anaerobes, strains B7-2T and B5T, were isolated from the Zoige Wetland on the Qinghai-Tibetan Plateau. The 16S rRNA gene sequences of strains B7-2T and B5T shared high similarity (>99â%) with those of the type strains of the genus Trichococcus, while their digital DNA-DNA hybridization values with each other (49â%) and with the reference type strains (48-23â%) were lower than 70â%, which suggest that they represent two novel species of the genus Trichococcus. Cells of strains B7-2T and B5T were immotile cocci, grew in the temperature range of 4-37 °C (optimum 25 °C) and were alkaliphilic with optimum growth at pH 9.0. The major components of the cellular fatty acids were C16ââ0, anteiso-C17ââ0 and C18ââ0 for strain B7-2T, and C16ââ0, anteiso-C17ââ0, C18ââ1ω9c and C18ââ0 for strain B5T. The genomic DNA G+C contents were 46.0 and 46.7 mol% for strains B7-2T and B5T, respectively. Based on physiological and genomic characteristics, it is suggested that strains B7-2T and B5T represent two novel species within the genus Trichococcus, for which the names Trichococcus paludicola sp. nov. and Trichococcus alkaliphilus sp. nov. are proposed. The type strains are B7-2T (=DSM 104691T=KCTC 33886T) and B5T (=DSM 104692T=KCTC 33885T), respectively.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phylogeny
/
Wetlands
/
Carnobacteriaceae
Country/Region as subject:
Asia
Language:
En
Journal:
Int J Syst Evol Microbiol
Journal subject:
MICROBIOLOGIA
Year:
2018
Type:
Article