[Experimental study of glioma stem cell-mediated immune tolerance in tumor microenvironment].
Zhonghua Zhong Liu Za Zhi
; 39(11): 808-813, 2017 Nov 23.
Article
in Zh
| MEDLINE
| ID: mdl-29151286
ABSTRACT
Objective:
To investigate the tumor microenvironment of immune tolerance induced by glioma stem cells (GSC).Methods:
Human GSC SU3 cells transfected with red fluorescent protein (SU3-RFP) gene were implanted into the brain, subcutis (armpit and foot), liver and abdominal cavity of transgenic green fluorescence protein (GFP) nude mice to establish RFP(+) /GFP(+) dual fluorescence solid tumor model. The re-cultured cells derived from implanted tumor tissues, SU3-RFP cells co-cultured with peritoneal fluid of transgenic GFP nude mice and malignant ascites of tumor-bearing mice were observed by fluorescence microscopy and real-time video image tracing to analyze the microenvironment of immune tolerance mediated by RFP(+) /GFP(+) implanted tumor.Results:
Dual fluorescence labeled frozen section showed that all of cells in the tumor microenvironment were GFP(+) , while the pressed tissue-patch showed that the tumor blood vessels exhibited a RFP(+) /GFP(+) double-positioning yellow. In the GFP single fluorescence labeled tumor tissue, all of cells in the microenvironment were green, including tumor edge, necrotic foci and blood vessel. Among them, CD68(+) , F4/80(+) , CD11c(+) , CD11b(+) and CD80(+) cells were observed. In the dual fluorescence labeled co-cultured cells, the phagocytosis and fusion between green host cells and red tumor cells were also observed, and these fusion cells might transfer to the malignant dendritic cells and macrophages.Conclusions:
The tumor microenvironment of immune tolerance induced by GSC is not affected by the tissue types of tumor-inoculated sites, and the immune tolerance mediated by inflammatory cells is associated with the inducible malignant transformation, which may be driven by cell fusion.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Neoplastic Stem Cells
/
Brain Neoplasms
/
Tumor Microenvironment
/
Glioma
/
Immune Tolerance
/
Immunity, Cellular
Limits:
Animals
/
Humans
Language:
Zh
Journal:
Zhonghua Zhong Liu Za Zhi
Year:
2017
Type:
Article
Affiliation country:
China