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Thrombospondins: Purification of human platelet thrombospondin-1.
Sipes, John M; Murphy-Ullrich, Joanne E; Roberts, David D.
Affiliation
  • Sipes JM; Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, United States.
  • Murphy-Ullrich JE; University of Alabama at Birmingham, Birmingham, AL, United States. Electronic address: jmurphy@uabmc.edu.
  • Roberts DD; Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, United States. Electronic address: droberts@helix.nih.gov.
Methods Cell Biol ; 143: 347-369, 2018.
Article in En | MEDLINE | ID: mdl-29310787
Thrombospondins are a family of five secreted proteins that have diverse roles in modulating cellular function. Thrombospondins-1 and 2 were identified as matricellular proteins based on their functional roles combined with their transient appearance or accumulation in extracellular matrix at specific times during development and in response to injury or stress in mature tissues. Thrombospondin-1 is a major component of platelet α-granules, which provides a convenient source for purification of the protein. Methods are described to prepare thrombospondin-1 from human platelets in a biologically active form with minimal degradation or contamination with other platelet proteins. A nondenaturing method is described for removing bound transforming growth factor-ß1.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Blood Platelets / Chromatography, Affinity / Thrombospondin 1 / Extracellular Matrix Type of study: Prognostic_studies Limits: Humans Language: En Journal: Methods Cell Biol Year: 2018 Type: Article Affiliation country: United States

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Blood Platelets / Chromatography, Affinity / Thrombospondin 1 / Extracellular Matrix Type of study: Prognostic_studies Limits: Humans Language: En Journal: Methods Cell Biol Year: 2018 Type: Article Affiliation country: United States