Thrombospondins: Purification of human platelet thrombospondin-1.
Methods Cell Biol
; 143: 347-369, 2018.
Article
in En
| MEDLINE
| ID: mdl-29310787
Thrombospondins are a family of five secreted proteins that have diverse roles in modulating cellular function. Thrombospondins-1 and 2 were identified as matricellular proteins based on their functional roles combined with their transient appearance or accumulation in extracellular matrix at specific times during development and in response to injury or stress in mature tissues. Thrombospondin-1 is a major component of platelet α-granules, which provides a convenient source for purification of the protein. Methods are described to prepare thrombospondin-1 from human platelets in a biologically active form with minimal degradation or contamination with other platelet proteins. A nondenaturing method is described for removing bound transforming growth factor-ß1.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Blood Platelets
/
Chromatography, Affinity
/
Thrombospondin 1
/
Extracellular Matrix
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
Methods Cell Biol
Year:
2018
Type:
Article
Affiliation country:
United States