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Sulfinate Based Selective Labeling of 5-Hydroxymethylcytosine: Application to Biotin Pull Down Assay.
Wu, Qiong; Amrutkar, Suyog Madhav; Shao, Fangwei.
Affiliation
  • Wu Q; Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University , 21 Nanyang Link, Singapore , 637371.
  • Amrutkar SM; Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University , 21 Nanyang Link, Singapore , 637371.
  • Shao F; Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University , 21 Nanyang Link, Singapore , 637371.
Bioconjug Chem ; 29(2): 245-249, 2018 02 21.
Article in En | MEDLINE | ID: mdl-29382202
ABSTRACT
We developed an enzyme-free, chemical method to selectively label the epigenetic base, 5-hydroxymethylcytosine (hmC) with versatile sulfinate reagents in aqueous solvent under mild reaction conditions. This method allows efficient single step conjugation of biotin to hmC site in DNA for enrichment and pull down assays.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Biotin / DNA / Biotinylation / 5-Methylcytosine Language: En Journal: Bioconjug Chem Journal subject: BIOQUIMICA Year: 2018 Type: Article
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Biotin / DNA / Biotinylation / 5-Methylcytosine Language: En Journal: Bioconjug Chem Journal subject: BIOQUIMICA Year: 2018 Type: Article