Vector competence of Culex antennatus and Anopheles coustani mosquitoes for Rift Valley fever virus in Madagascar.
Med Vet Entomol
; 32(2): 259-262, 2018 06.
Article
in En
| MEDLINE
| ID: mdl-29383746
ABSTRACT
Culex antennatus (Diptera Culicidae), Anopheles coustani (Diptera Culicidae) and Anopheles squamosus/cydippis were found to be infected with Rift Valley fever virus (RVFV) during an epidemic that occurred in 2008 and 2009 in Madagascar. To understand the roles played by Cx. antennatus and An. coustani in virus maintenance and transmission, RVFV vector competence was assessed in each species. Mosquito body parts and saliva of mosquitoes that fed on RVFV-infected blood were tested for RVFV using real-time quantitative polymerase chain reaction (RT-qPCR) assays. Overall, viral RNA was detected in body parts and saliva at 5 days post-infection (d.p.i.) in both species. At 5 d.p.i., infection rates were 12.5% (3/24) and 15.8% (6/38), disseminated infection rates were 100% (3/3) and 100% (6/6), transmission rates were 33.3% (1/3) and 83.3% (5/6), and transmission efficiencies were 4.2% (1/24) and 13.2% (5/38) in Cx. antennatus and An. coustani, respectively. Although RVFV detected in saliva did not propagate on to Vero cells, these results support potential roles for these two mosquito species in the transmission of RVFV.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Rift Valley Fever
/
Rift Valley fever virus
/
Culex
/
Mosquito Vectors
/
Anopheles
Type of study:
Risk_factors_studies
Limits:
Animals
Country/Region as subject:
Africa
Language:
En
Journal:
Med Vet Entomol
Journal subject:
BIOLOGIA
/
MEDICINA VETERINARIA
Year:
2018
Type:
Article
Affiliation country:
Madagascar